<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Characterization of Molecules Involved in Protein Translocation Using a Specific Antibody"^^ . "The vectorial translocation of nascent proteins through the membrane of the rough endoplasmic reticulum has been shown to require a specific membrane-bound protein whose cytoplasmic domain can be proteolytically cleaved and isolated as an active peptide of mol wt 60,000 (Meyer and Dobberstein, 1980, J. Cell Biol. 87:503-508). Rabbit antibodies raised against this peptide were used to further characterize the membrane-bound molecule. Immunoprecipitation of solubilized, radiolabeled rough microsomal proteins yielded a single polypeptide of mol wt 72,000, representing the membrane-bound protein from which the 60,000-mol wt peptide was proteolytically derived . The antibody could also be used to remove exclusively the 60,000-mol wt peptide, and thus the translocation activity, from elastase digests tested in a reconstituted system. Moreover,immunoprecipitation of elastase extracts alkylated with [t4C] N-ethylmaleimide selected a single species of mol wt 60,000. Immunoprecipitation of in vivo radiolabeled proteins from the appropriate cell type yielded the 72,000-mol wt membrane protein irrespective of the duration of labeling, or if followed by a chase. Subsequent treatment with protease generated the 60,000-mol wt fragment. In addition, the antibody could be used to visualize reticular structures in intact cells which correspond to endoplasmic reticulum at the ultrastructural level . It is thus clear that one membrane component required in the vectorial translocation of nascent secretory (and membrane) proteins is a peptide of mol wt 72,000."^^ . "1982" . . . "92" . . "The Journal of Cell Biology"^^ . . . . . . . . . . . . . "Bernhard"^^ . "Dobberstein"^^ . "Bernhard Dobberstein"^^ . . "David I."^^ . "Meyer"^^ . "David I. Meyer"^^ . . "Daniel"^^ . "Louvard"^^ . "Daniel Louvard"^^ . . . . . . "Characterization of Molecules Involved in Protein Translocation Using a Specific Antibody (PDF)"^^ . . . "Meyer_1982.JCB.pdf"^^ . . . "Characterization of Molecules Involved in Protein Translocation Using a Specific Antibody (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Characterization of Molecules Involved in Protein Translocation Using a Specific Antibody (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Characterization of Molecules Involved in Protein Translocation Using a Specific Antibody (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Characterization of Molecules Involved in Protein Translocation Using a Specific Antibody (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Characterization of Molecules Involved in Protein Translocation Using a Specific Antibody (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #8552 \n\nCharacterization of Molecules Involved in Protein Translocation Using a Specific Antibody\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .