eprintid: 8554
rev_number: 4
eprint_status: archive
userid: 1
dir: disk0/00/00/85/54
datestamp: 2008-07-15 17:23:25
lastmod: 2008-07-15 17:23:25
status_changed: 2012-08-16 08:07:38
type: article
metadata_visibility: show
creators_name: Zachgo, Sabine
creators_name: Dobberstein, Bernhard
creators_name: Griffiths, Gareth
title: A block in degradation of MHC class II-associated invariant chain correlates with a reduction in transport from endosome carrier vesicles to the prelysosome compartment
ispublished: pub
subjects: ddc-570
divisions: i-706000
keywords: leupeptin , invariant chain (Ii) , endosome carrier vesicle (ECV) , endocytosis
abstract: Invariant chain (Ii) associated with MHC class II molecule is processed proteolytically via several distinct intermediates during its intracellular transport through endosomal compartments. Leupeptin added to the culture medium blocks processing of Ii, prevents its dissociation from the class II molecules and leads to an intracellular accumulation of a 22 kDa intermediate form of Ii. We show here that leupeptin has a very general effect on protein transport in the endocytic pathway.  When added to Mel Juso cells leupeptin reduces the transport of endocytosed material from multivesicular body-like, endosome carrier vesicles (ECV) to the prelysosomal compartment (late endosome) and leads to a concomitant increase in the number of ECV. Our results argue that one effect of leupeptin, related to antigen processing and presentation, is to block transport of antigen and/or MHC class II molecules to prelysosomal compartments.
abstract_translated_lang: eng
date: 1992
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008554
ppn_swb: 326751947
own_urn: urn:nbn:de:bsz:16-opus-85544
language: eng
bibsort: ZACHGOSABIABLOCKINDE1992
full_text_status: none
publication: Journal of Cell Science
volume: 103
pagerange: 811-822
citation:   Zachgo, Sabine ; Dobberstein, Bernhard ; Griffiths, Gareth  (1992) A block in degradation of MHC class II-associated invariant chain correlates with a reduction in transport from endosome carrier vesicles to the prelysosome compartment.  Journal of Cell Science, 103.  pp. 811-822.