title: The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences
creator: Lütcke, Henrich
creator: High, Stephen
creator: Römisch, Karin
creator: Ashford, Anthony J.
creator: Dobberstein, Bernhard
subject: 570
subject: 570 Life sciences
description: The signal recognition particle (SRP) binds to signal sequences when they emerge from a translating ribosome and targets the complex of ribosome, nascent chain and SRP to the membrane of the rough endoplasmic reticulum (rER) allowing the co-translational translocation of the nascent chain.  By photo-crosslinking it has been shown that the signal sequence of preprolactin(PPL) only interacts with the methionine-rich (NI) domain of the 54 kDa protein subunit (SRP54) of SRP. Here we show that (i) a signal-anchor sequence is likewise crosslinked only to the methionine-rich domain of SRP54,(ii) free SRP54 can interact with signal sequences independently of the other components of SRP, (iii) its M domain suffices to perform this function, and (iv) an essentially intact M domain is required for signal sequence recognition. Alkylation of the N+G domain in intact SRP54 with N-ethyl maleimide (NE), but not after cleavage with V8 protease, prevents the binding of a signal sequence to the M domain. This suggests a proximity between the N+G and M domains of SRP54 and raises the possibility that the role of the N+G domain may be to regulate the binding and/or the release of signal sequences.
date: 1992
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8555/1/Luetcke_1992_EMBOJ.pdf
identifier: DOI:10.11588/heidok.00008555
identifier: urn:nbn:de:bsz:16-opus-85553
identifier:   Lütcke, Henrich ; High, Stephen ; Römisch, Karin ; Ashford, Anthony J. ; Dobberstein, Bernhard  (1992) The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences.  The EMBO Journal, 11 (4).  pp. 1543-1551.      
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8555/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng