<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences"^^ . "The signal recognition particle (SRP) binds to signal sequences when they emerge from a translating ribosome and targets the complex of ribosome, nascent chain and SRP to the membrane of the rough endoplasmic reticulum (rER) allowing the co-translational translocation of the nascent chain. By photo-crosslinking it has been shown that the signal sequence of preprolactin(PPL) only interacts with the methionine-rich (NI) domain of the 54 kDa protein subunit (SRP54) of SRP. Here we show that (i) a signal-anchor sequence is likewise crosslinked only to the methionine-rich domain of SRP54,(ii) free SRP54 can interact with signal sequences independently of the other components of SRP, (iii) its M domain suffices to perform this function, and (iv) an essentially intact M domain is required for signal sequence recognition. Alkylation of the N+G domain in intact SRP54 with N-ethyl maleimide (NE), but not after cleavage with V8 protease, prevents the binding of a signal sequence to the M domain. This suggests a proximity between the N+G and M domains of SRP54 and raises the possibility that the role of the N+G domain may be to regulate the binding and/or the release of signal sequences."^^ . "1992" . . . "11" . "4" . . "The EMBO Journal"^^ . . . . . . . . . . . . . . . . . . . "Bernhard"^^ . "Dobberstein"^^ . "Bernhard Dobberstein"^^ . . "Stephen"^^ . "High"^^ . "Stephen High"^^ . . "Karin"^^ . "Römisch"^^ . "Karin Römisch"^^ . . "Henrich"^^ . "Lütcke"^^ . "Henrich Lütcke"^^ . . "Anthony J."^^ . "Ashford"^^ . "Anthony J. Ashford"^^ . . . . . . "The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences (PDF)"^^ . . . "Luetcke_1992_EMBOJ.pdf"^^ . . . "The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences (Other)"^^ . . . . . . "preview.jpg"^^ . . . "The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences (Other)"^^ . . . . . . "medium.jpg"^^ . . . "The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #8555 \n\nThe methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .