title: Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone
creator: Singer, Paul A.
creator: Lauer, Wolfgang
creator: Dembic, Zlatko
creator: Mayer, Werner E.
creator: Lipp, Joachim
creator: Koch, Norbert
creator: Hämmerling, Günter
creator: Klein, Jan
creator: Dobberstein, Bernhard
subject: 570
subject: 570 Life sciences
description: The invariant (Ii) chain is a membrane-spanning glycoprotein found intracellularly associated with class II major histocompatibility complex (MHC) molecules. Using hybridselected translation and the Ii-specific monoclonal antibody In-1, we have isolated a cDNA clone (pli-5) coding for most of the Ii chain. Sequence analysis of this clone reveals an open reading frame encoding 169 amino acid residues. The protein is rich in methionine and contains two potential N-glycosylation sites. No stretch of uncharged amino acid residues, characteristic for a membrane-spanning segment, is found close to the COOH-terminal end. There is one, however, close to the NH2-terminal end. As it is known that -20 amino acid residues of Ii chain are exposed on the cytoplasmic side, we conclude that the Ii chain spans the membrane exposing the NH2 terminus on the cytoplasmic side and the COOH terminus on the luminal side. 
date: 1984
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8561/1/Singer_1984_EMBOJ.pdf
identifier: DOI:10.11588/heidok.00008561
identifier: urn:nbn:de:bsz:16-opus-85616
identifier:   Singer, Paul A. ; Lauer, Wolfgang ; Dembic, Zlatko ; Mayer, Werner E. ; Lipp, Joachim ; Koch, Norbert ; Hämmerling, Günter ; Klein, Jan ; Dobberstein, Bernhard  (1984) Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone.  The EMBO Journal, 3 (4).  pp. 873-877.      
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8561/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng