TY  - JOUR
KW  - major histocompatibility complex 
KW  -  invariant chain 
KW  -  cDNA cloning 
KW  -  transmembrane proteins
AV  - public
ID  - heidok8561
SP  - 873
VL  - 3
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/8561/
EP  - 877
A1  - Singer, Paul A.
A1  - Lauer, Wolfgang
A1  - Dembic, Zlatko
A1  - Mayer, Werner E.
A1  - Lipp, Joachim
A1  - Koch, Norbert
A1  - Hämmerling, Günter
A1  - Klein, Jan
A1  - Dobberstein, Bernhard
N2  - The invariant (Ii) chain is a membrane-spanning glycoprotein found intracellularly associated with class II major histocompatibility complex (MHC) molecules. Using hybridselected translation and the Ii-specific monoclonal antibody In-1, we have isolated a cDNA clone (pli-5) coding for most of the Ii chain. Sequence analysis of this clone reveals an open reading frame encoding 169 amino acid residues. The protein is rich in methionine and contains two potential N-glycosylation sites. No stretch of uncharged amino acid residues, characteristic for a membrane-spanning segment, is found close to the COOH-terminal end. There is one, however, close to the NH2-terminal end. As it is known that -20 amino acid residues of Ii chain are exposed on the cytoplasmic side, we conclude that the Ii chain spans the membrane exposing the NH2 terminus on the cytoplasmic side and the COOH terminus on the luminal side. 
TI  - Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone
JF  - The EMBO Journal
IS  - 4
Y1  - 1984///
ER  -