eprintid: 8561
rev_number: 16
eprint_status: archive
userid: 1
dir: disk0/00/00/85/61
datestamp: 2008-07-17 15:23:52
lastmod: 2015-12-30 03:37:47
status_changed: 2012-08-14 15:25:55
type: article
metadata_visibility: show
creators_name: Singer, Paul A.
creators_name: Lauer, Wolfgang
creators_name: Dembic, Zlatko
creators_name: Mayer, Werner E.
creators_name: Lipp, Joachim
creators_name: Koch, Norbert
creators_name: Hämmerling, Günter
creators_name: Klein, Jan
creators_name: Dobberstein, Bernhard
title: Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone
ispublished: pub
subjects: 570
divisions: 706000
keywords: major histocompatibility complex , invariant chain , cDNA cloning , transmembrane proteins
abstract: The invariant (Ii) chain is a membrane-spanning glycoprotein found intracellularly associated with class II major histocompatibility complex (MHC) molecules. Using hybridselected translation and the Ii-specific monoclonal antibody In-1, we have isolated a cDNA clone (pli-5) coding for most of the Ii chain. Sequence analysis of this clone reveals an open reading frame encoding 169 amino acid residues. The protein is rich in methionine and contains two potential N-glycosylation sites. No stretch of uncharged amino acid residues, characteristic for a membrane-spanning segment, is found close to the COOH-terminal end. There is one, however, close to the NH2-terminal end. As it is known that -20 amino acid residues of Ii chain are exposed on the cytoplasmic side, we conclude that the Ii chain spans the membrane exposing the NH2 terminus on the cytoplasmic side and the COOH terminus on the luminal side. 
abstract_translated_lang: eng
date: 1984
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008561
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 028
ppn_swb: 1354115236
own_urn: urn:nbn:de:bsz:16-opus-85616
language: eng
bibsort: SINGERPAULSTRUCTUREO1984
full_text_status: public
publication: The EMBO Journal
volume: 3
number: 4
pagerange: 873-877
citation:   Singer, Paul A. ; Lauer, Wolfgang ; Dembic, Zlatko ; Mayer, Werner E. ; Lipp, Joachim ; Koch, Norbert ; Hämmerling, Günter ; Klein, Jan ; Dobberstein, Bernhard  (1984) Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone.  The EMBO Journal, 3 (4).  pp. 873-877.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8561/1/Singer_1984_EMBOJ.pdf