TY  - JOUR
Y1  - 1993///
EP  - 750
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/8568/
JF  - The Journal of Cell Biology
ID  - heidok8568
N2  - We have identified membrane components which are adjacent to type I and type II signal-anchor proteins during their insertion into the membrane of the ER. Using two different cross-linking approaches a 37-38-kD nonglycosylated protein, previously identified as P37 (High, S., D. G6dich, M. Wiedmann, T. A. Rapoport, and B. Dobberstein. 1991. J. Cell Biol. 113:35-44), was found adjacent to all the membrane inserted nascent chains used in this study. On the basis of immunoprecipitation, this ER protein was shown to be identical to the recently identified mammalian Sec61 protein. Thus, Sec61p is the principal cross-linking partner of both type I and type II signal-anchor proteins during their membrane insertion (this work), and of secretory proteins during their translocation (G-tdich, D., S. Prehn, E. Hartmann, K.-U. Kalies, and T. A. Rapoport. 1992. Cell. 71:489-503). We propose that membrane proteins of both orientations, and secretory proteins employ the same ER translocation sites, and that Sec61p is a core component of these sites.
VL  - 121
AV  - public
IS  - 4
TI  - Sec61p is Adjacent to Nascent Type I and Type II Signal-Anchor Proteins during Their Membrane Insertion
SP  - 743
A1  - High, Stephen
A1  - Andersen, Soren S. L.
A1  - Görlich, Dirk
A1  - Hartmann, Enno
A1  - Prehn, Siegfried
A1  - Rapoport, Tom A.
A1  - Dobberstein, Bernhard
ER  -