eprintid: 8568
rev_number: 8
eprint_status: archive
userid: 1
dir: disk0/00/00/85/68
datestamp: 2008-07-17 17:08:58
lastmod: 2014-08-20 20:59:59
status_changed: 2012-08-14 15:25:55
type: article
metadata_visibility: show
creators_name: High, Stephen
creators_name: Andersen, Soren S. L.
creators_name: Görlich, Dirk
creators_name: Hartmann, Enno
creators_name: Prehn, Siegfried
creators_name: Rapoport, Tom A.
creators_name: Dobberstein, Bernhard
title: Sec61p is Adjacent to Nascent Type I and Type II Signal-Anchor Proteins during Their Membrane Insertion
ispublished: pub
subjects: 570
divisions: 706000
abstract: We have identified membrane components which are adjacent to type I and type II signal-anchor proteins during their insertion into the membrane of the ER. Using two different cross-linking approaches a 37-38-kD nonglycosylated protein, previously identified as P37 (High, S., D. G6dich, M. Wiedmann, T. A. Rapoport, and B. Dobberstein. 1991. J. Cell Biol. 113:35-44), was found adjacent to all the membrane inserted nascent chains used in this study. On the basis of immunoprecipitation, this ER protein was shown to be identical to the recently identified mammalian Sec61 protein. Thus, Sec61p is the principal cross-linking partner of both type I and type II signal-anchor proteins during their membrane insertion (this work), and of secretory proteins during their translocation (G-tdich, D., S. Prehn, E. Hartmann, K.-U. Kalies, and T. A. Rapoport. 1992. Cell. 71:489-503). We propose that membrane proteins of both orientations, and secretory proteins employ the same ER translocation sites, and that Sec61p is a core component of these sites.
abstract_translated_lang: eng
date: 1993
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008568
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 064
ppn_swb: 1354117859
own_urn: urn:nbn:de:bsz:16-opus-85689
language: eng
bibsort: HIGHSTEPHESEC61PISAD1993
full_text_status: public
publication: The Journal of Cell Biology
volume: 121
number: 4
pagerange: 743-750
citation:   High, Stephen ; Andersen, Soren S. L. ; Görlich, Dirk ; Hartmann, Enno ; Prehn, Siegfried ; Rapoport, Tom A. ; Dobberstein, Bernhard  (1993) Sec61p is Adjacent to Nascent Type I and Type II Signal-Anchor Proteins during Their Membrane Insertion.  The Journal of Cell Biology, 121 (4).  pp. 743-750.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8568/1/High_1993_JCB.pdf