title: Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence
creator: High, Stephen
creator: Martoglio, Bruno
creator: Görlich, Dirk
creator: Andersen, Soren S. L.
creator: Ashford, Anthony J.
creator: Giner, Angelika
creator: Hartmann, Enno
creator: Prehn, Siegfried
creator: Rapoport, Tom A.
creator: Dobberstein, Bernhard
creator: Brunner, Josef
subject: 570
subject: 570 Life sciences
description: A chemically charged amber suppressor tRNA was used to introduce the photoactivatable amino acid (Tmd)Phe at a selected position within the signal sequence of the secretory protein preprolactin. This allowed the interactions of the NH -terminal, the central, and the COOH-terminal regions of the signal sequence to be investigated during insertion into the membrane of the endoplasmic reticulum (ER). We found that different regions of the nascent chains were photocross-linked to different ER proteins. The TRAM protein (translocating chain-associating membrane protein) contacts the NHz-terminal region of the signal sequence while the mammalian Sec6lp contacts the hydrophobic core of the signal sequence and regions COOH-terminal of this. These results suggest that the ER translocation complex is composed of heterologous protein subunits which contadcti stinct regions of nascent polypeptides during their membrane insertion.
date: 1993
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8569/1/High_1993_JBC.pdf
identifier: DOI:10.11588/heidok.00008569
identifier: urn:nbn:de:bsz:16-opus-85698
identifier:   High, Stephen ; Martoglio, Bruno ; Görlich, Dirk ; Andersen, Soren S. L. ; Ashford, Anthony J. ; Giner, Angelika ; Hartmann, Enno ; Prehn, Siegfried ; Rapoport, Tom A. ; Dobberstein, Bernhard ; Brunner, Josef  (1993) Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence.  The Journal of Biological Chemistry, 268 (35).  pp. 26746-2671.      
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8569/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng