<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence"^^ . "A chemically charged amber suppressor tRNA was used to introduce the photoactivatable amino acid (Tmd)Phe at a selected position within the signal sequence of the secretory protein preprolactin. This allowed the interactions of the NH -terminal, the central, and the COOH-terminal regions of the signal sequence to be investigated during insertion into the membrane of the endoplasmic reticulum (ER). We found that different regions of the nascent chains were photocross-linked to different ER proteins. The TRAM protein (translocating chain-associating membrane protein) contacts the NHz-terminal region of the signal sequence while the mammalian Sec6lp contacts the hydrophobic core of the signal sequence and regions COOH-terminal of this. These results suggest that the ER translocation complex is composed of heterologous protein subunits which contadcti stinct regions of nascent polypeptides during their membrane insertion."^^ . "1993" . . . "268" . "35" . . "The Journal of Biological Chemistry"^^ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Stephen"^^ . "High"^^ . "Stephen High"^^ . . "Tom A."^^ . "Rapoport"^^ . "Tom A. Rapoport"^^ . . "Bernhard"^^ . "Dobberstein"^^ . "Bernhard Dobberstein"^^ . . "Soren S. L."^^ . "Andersen"^^ . "Soren S. L. Andersen"^^ . . "Siegfried"^^ . "Prehn"^^ . "Siegfried Prehn"^^ . . "Angelika"^^ . "Giner"^^ . "Angelika Giner"^^ . . "Josef"^^ . "Brunner"^^ . "Josef Brunner"^^ . . "Dirk"^^ . "Görlich"^^ . "Dirk Görlich"^^ . . "Anthony J."^^ . "Ashford"^^ . "Anthony J. Ashford"^^ . . "Enno"^^ . "Hartmann"^^ . "Enno Hartmann"^^ . . "Bruno"^^ . "Martoglio"^^ . "Bruno Martoglio"^^ . . . . . . "Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence (PDF)"^^ . . . "High_1993_JBC.pdf"^^ . . . "Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #8569 \n\nSite-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .