TY  - JOUR
Y1  - 1993///
IS  - 35
A1  - High, Stephen
A1  - Martoglio, Bruno
A1  - Görlich, Dirk
A1  - Andersen, Soren S. L.
A1  - Ashford, Anthony J.
A1  - Giner, Angelika
A1  - Hartmann, Enno
A1  - Prehn, Siegfried
A1  - Rapoport, Tom A.
A1  - Dobberstein, Bernhard
A1  - Brunner, Josef
N2  - A chemically charged amber suppressor tRNA was used to introduce the photoactivatable amino acid (Tmd)Phe at a selected position within the signal sequence of the secretory protein preprolactin. This allowed the interactions of the NH -terminal, the central, and the COOH-terminal regions of the signal sequence to be investigated during insertion into the membrane of the endoplasmic reticulum (ER). We found that different regions of the nascent chains were photocross-linked to different ER proteins. The TRAM protein (translocating chain-associating membrane protein) contacts the NHz-terminal region of the signal sequence while the mammalian Sec6lp contacts the hydrophobic core of the signal sequence and regions COOH-terminal of this. These results suggest that the ER translocation complex is composed of heterologous protein subunits which contadcti stinct regions of nascent polypeptides during their membrane insertion.
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/8569/
TI  - Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence
SP  - 26746
JF  - The Journal of Biological Chemistry
ID  - heidok8569
AV  - public
VL  - 268
EP  - 2671
ER  -