eprintid: 8569
rev_number: 8
eprint_status: archive
userid: 1
dir: disk0/00/00/85/69
datestamp: 2008-07-17 17:10:43
lastmod: 2014-08-20 18:45:54
status_changed: 2012-08-14 15:25:55
type: article
metadata_visibility: show
creators_name: High, Stephen
creators_name: Martoglio, Bruno
creators_name: Görlich, Dirk
creators_name: Andersen, Soren S. L.
creators_name: Ashford, Anthony J.
creators_name: Giner, Angelika
creators_name: Hartmann, Enno
creators_name: Prehn, Siegfried
creators_name: Rapoport, Tom A.
creators_name: Dobberstein, Bernhard
creators_name: Brunner, Josef
title: Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence
ispublished: pub
subjects: 570
divisions: 706000
abstract: A chemically charged amber suppressor tRNA was used to introduce the photoactivatable amino acid (Tmd)Phe at a selected position within the signal sequence of the secretory protein preprolactin. This allowed the interactions of the NH -terminal, the central, and the COOH-terminal regions of the signal sequence to be investigated during insertion into the membrane of the endoplasmic reticulum (ER). We found that different regions of the nascent chains were photocross-linked to different ER proteins. The TRAM protein (translocating chain-associating membrane protein) contacts the NHz-terminal region of the signal sequence while the mammalian Sec6lp contacts the hydrophobic core of the signal sequence and regions COOH-terminal of this. These results suggest that the ER translocation complex is composed of heterologous protein subunits which contadcti stinct regions of nascent polypeptides during their membrane insertion.
abstract_translated_lang: eng
date: 1993
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008569
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 065
ppn_swb: 1354118529
own_urn: urn:nbn:de:bsz:16-opus-85698
language: eng
bibsort: HIGHSTEPHESITESPECIF1993
full_text_status: public
publication: The Journal of Biological Chemistry
volume: 268
number: 35
pagerange: 26746-2671
citation:   High, Stephen ; Martoglio, Bruno ; Görlich, Dirk ; Andersen, Soren S. L. ; Ashford, Anthony J. ; Giner, Angelika ; Hartmann, Enno ; Prehn, Siegfried ; Rapoport, Tom A. ; Dobberstein, Bernhard ; Brunner, Josef  (1993) Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence.  The Journal of Biological Chemistry, 268 (35).  pp. 26746-2671.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8569/1/High_1993_JBC.pdf