title: Assembly of the 68- and 72-kD Proteins of Signal Recognition Particle with 7S RNA
creator: Lütcke, Henrich
creator: Prehn, Siegfried
creator: Ashford, Anthony J.
creator: Remus, Michael
creator: Frank, Rainer
creator: Dobberstein, Bernhard
subject: 570
subject: 570 Life sciences
description: Signal recognition particle (SRP), the cytoplasmic ribonucleoprotein particle that mediates the targeting of proteins to the ER, consists of a 7S RNA and six different proteins. The 68- (SRP68) and 72-(SRP72) kD proteins of SRP are bound to the 7S RNA of SRP as a heterodimeric complex (SRP68/72). Here we describe the primary structure of SRP72 and the assembly of SRP68, SRP72 and 7S RNA into a ribonucleoprotein particle.  The amino acid sequence deduced from the cDNA of SRP72 reveals a basic protein of 671 amino acids which shares no sequence similarity with any protein in the sequence data libraries. Assembly of SRP72 into a ribonucleoprotein particle required the presence of 7S RNA and SRP68. In contrast, SRP68 alone specifically bound to 7S RNA. SRP68 contacts the 7S RNA via its NH2-terminal half while COOH-terminal portions of SRP68 and SRP72 are in contact with each other in SRP. SRP68 thus serves as a link between 7S RNA and SRP72. As a large NH2-terminal domain of SRP72 is exposed on SRP it may be a site of contact to other molecules involved in the SRP cycle between the ribosome and the ER membrane.
date: 1993
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8581/1/Luetcke_1993_JCB.pdf
identifier: DOI:10.11588/heidok.00008581
identifier: urn:nbn:de:bsz:16-opus-85813
identifier:   Lütcke, Henrich ; Prehn, Siegfried ; Ashford, Anthony J. ; Remus, Michael ; Frank, Rainer ; Dobberstein, Bernhard  (1993) Assembly of the 68- and 72-kD Proteins of Signal Recognition Particle with 7S RNA.  The Journal of Cell Biology, 121 (5).  pp. 977-985.      
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8581/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng