eprintid: 8581
rev_number: 8
eprint_status: archive
userid: 1
dir: disk0/00/00/85/81
datestamp: 2008-07-22 14:52:43
lastmod: 2014-08-29 09:08:37
status_changed: 2012-08-14 15:25:58
type: article
metadata_visibility: show
creators_name: Lütcke, Henrich
creators_name: Prehn, Siegfried
creators_name: Ashford, Anthony J.
creators_name: Remus, Michael
creators_name: Frank, Rainer
creators_name: Dobberstein, Bernhard
title: Assembly of the 68- and 72-kD Proteins of Signal Recognition Particle with 7S RNA
ispublished: pub
subjects: 570
divisions: 706000
abstract: Signal recognition particle (SRP), the cytoplasmic ribonucleoprotein particle that mediates the targeting of proteins to the ER, consists of a 7S RNA and six different proteins. The 68- (SRP68) and 72-(SRP72) kD proteins of SRP are bound to the 7S RNA of SRP as a heterodimeric complex (SRP68/72). Here we describe the primary structure of SRP72 and the assembly of SRP68, SRP72 and 7S RNA into a ribonucleoprotein particle.  The amino acid sequence deduced from the cDNA of SRP72 reveals a basic protein of 671 amino acids which shares no sequence similarity with any protein in the sequence data libraries. Assembly of SRP72 into a ribonucleoprotein particle required the presence of 7S RNA and SRP68. In contrast, SRP68 alone specifically bound to 7S RNA. SRP68 contacts the 7S RNA via its NH2-terminal half while COOH-terminal portions of SRP68 and SRP72 are in contact with each other in SRP. SRP68 thus serves as a link between 7S RNA and SRP72. As a large NH2-terminal domain of SRP72 is exposed on SRP it may be a site of contact to other molecules involved in the SRP cycle between the ribosome and the ER membrane.
abstract_translated_lang: eng
date: 1993
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008581
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 068
ppn_swb: 1354703774
own_urn: urn:nbn:de:bsz:16-opus-85813
language: eng
bibsort: LUTCKEHENRASSEMBLYOF1993
full_text_status: public
publication: The Journal of Cell Biology
volume: 121
number: 5
pagerange: 977-985
citation:   Lütcke, Henrich ; Prehn, Siegfried ; Ashford, Anthony J. ; Remus, Michael ; Frank, Rainer ; Dobberstein, Bernhard  (1993) Assembly of the 68- and 72-kD Proteins of Signal Recognition Particle with 7S RNA.  The Journal of Cell Biology, 121 (5).  pp. 977-985.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8581/1/Luetcke_1993_JCB.pdf