eprintid: 8592
rev_number: 8
eprint_status: archive
userid: 1
dir: disk0/00/00/85/92
datestamp: 2009-03-02 10:59:51
lastmod: 2014-08-19 03:35:00
status_changed: 2012-08-14 15:28:11
type: article
metadata_visibility: show
creators_name: Prehn, Siegfried
creators_name: Herz, Joachim
creators_name: Hartmann, Enno
creators_name: Kurzchalia, Teymuras V.
creators_name: Frank, Rainer
creators_name: Römisch, Karin
creators_name: Dobberstein, Bernhard
creators_name: Rapoport, Tom A.
title: Structure and biosynthesis of the signal-sequence receptor
ispublished: pub
subjects: ddc-570
divisions: i-706000
abstract: The signal-sequence receptor (SSR) has previously been shown to be a component of the environment which nascent polypeptides meet on passage through the endoplasmic reticulum (ER) membrane. We report here on the primary structure of the SSR as deduced from cDNA clones and from direct protein sequencing. The glycoprotein is synthesized with a cleavable amino-terminal signal sequence and contains only one classical membrane-spanning segment. Its insertion into the ER membrane during biosynthesis depends on the function of the signal-recognition particle. SSR shows a remarkable charge distribution with the amino terminus being highly negatively charged, and the cytoplasmic carboxyl terminus positively charged. The SSR can be phosphorylated in its cytoplasmic tail both in intact cells and in a cell-free system, suggesting a regulation of its function. The localization of the protein in the ER membrane was confirmed by immunofluorescence microscopy.
abstract_translated_lang: eng
date: 1990
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008592
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 051
ppn_swb: 1373873833
own_urn: urn:nbn:de:bsz:16-opus-85923
language: eng
bibsort: PREHNSIEGFSTRUCTUREA1990
full_text_status: public
publication: European Journal of Biochemistry
volume: 188
pagerange: 439-445
citation:   Prehn, Siegfried ; Herz, Joachim ; Hartmann, Enno ; Kurzchalia, Teymuras V. ; Frank, Rainer ; Römisch, Karin ; Dobberstein, Bernhard ; Rapoport, Tom A.  (1990) Structure and biosynthesis of the signal-sequence receptor.  European Journal of Biochemistry, 188.  pp. 439-445.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8592/1/Prehn_1990_EJB.pdf