<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Disassembly and domain structure of the proteins in the signal-recognition particle"^^ . "The signal-recognition particle (SRP) is a ribonucleoprotein (RNP) complex consisting of six different polypeptide chains and a 7SL RNA. It participates in initiating the translocation of proteins across the membrane of the endoplasmic reticulum, SRP was disassembled in 2 M KCl into three components, one RNP composed of 7SL RNA and the 54-kDa and 19-kDa proteins, and two heterodimers consisting of the 72/68-kDa and the 14/9-kDa proteins respectively. The 54-kDa protein could be released from the RNP subparticle by chromatography on DEAE-Sepharose in Mg2 ’ -depleted buffer, while the 19-kDa protein remained bound to the 7SL RNA. The domain structure of SRP proteins was probed by using mild elastase treatment and protein-specific antibodies. It was found that the 72, 68, 54 and 19-kDa SRP proteins were proteolytically processed in distinct steps. Most remarkably a protein fragment of 55-kDa, generated from the 72-kDa SRP protein, and a 35-kDa fragment from the 54-kDa SRP protein were both released from the RNP particle. Fragments generated from the 68-kDa protein and detectable with the anti-(68-kDa protein) antibody remained associated with the RNP particle. Cleavage of the SRP proteins by elastase at 2.5 pg/ml resulted in partial loss of activity, while 10 lg/ml caused complete inactivation of the particle. Neither the elongation arrest of IgG light chain nor its translocation across SRP-depleted microsomal membranes was promoted. The implications of these results on the possible interaction between the SRP subunits are discussed."^^ . "1987" . . . "1987" . "163" . . "European Journal of Biochemistry"^^ . . . . . . . . . . . . . . . . "Bernhard"^^ . "Dobberstein"^^ . "Bernhard Dobberstein"^^ . . "Klaus"^^ . "Meese"^^ . "Klaus Meese"^^ . . "Elke"^^ . "Krause"^^ . "Elke Krause"^^ . . "Efstathia"^^ . "Scoulica"^^ . "Efstathia Scoulica"^^ . . . . . . "Disassembly and domain structure of the proteins in the signal-recognition particle (PDF)"^^ . . . "Scoulica_1987_EJB.pdf"^^ . . . "Disassembly and domain structure of the proteins in the signal-recognition particle (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Disassembly and domain structure of the proteins in the signal-recognition particle (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Disassembly and domain structure of the proteins in the signal-recognition particle (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Disassembly and domain structure of the proteins in the signal-recognition particle (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Disassembly and domain structure of the proteins in the signal-recognition particle (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #8593 \n\nDisassembly and domain structure of the proteins in the signal-recognition particle\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .