title: A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences
creator: Stüber, Dietrich
creator: Ibrahimi, Ibrahim
creator: Cutler, Daniel
creator: Dobberstein, Bernhard
creator: Bujard, Herrmann
subject: ddc-570
subject: 570 Life sciences
description: A system is described which permits the efficient synthesis of single proteins in vitro. The essential element in this expression system is a strong promoter derived from coliphage T5 which produces, with high efficiency, specific RNAs in capped or uncapped form, depending upon the experimental conditions used. The transcription-coupled capping of RNA allows the direct translation of the RNA in eukaryotic extracts from wheat germ as well as from HeLa cells. The synthesis of three different proteins is reported, including lysozyme, which is shown to be translocated across membranes when appropriate assay conditions are used. The simplicity of the experimental procedure, the high purity and specific activity of the [35S]methionine-labefled proteins produced offer a number of possibilities for the study of structure-function relationships of proteins.
date: 1984
type: Article
type: info:eu-repo/semantics/article
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8594/1/Stueber_1984_EMBOJ.pdf
identifier: DOI:10.11588/heidok.00008594
identifier: urn:nbn:de:bsz:16-opus-85945
identifier:   Stüber, Dietrich ; Ibrahimi, Ibrahim ; Cutler, Daniel ; Dobberstein, Bernhard ; Bujard, Herrmann  (1984) A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.  The EMBO Journal, 3 (13).  pp. 3143-3148.      
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8594/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng