eprintid: 8595
rev_number: 14
eprint_status: archive
userid: 1
dir: disk0/00/00/85/95
datestamp: 2009-03-02 11:01:59
lastmod: 2016-01-01 12:11:36
status_changed: 2012-08-14 15:28:11
type: article
metadata_visibility: show
creators_name: Xin, J.-H.
creators_name: Kvist, S.
creators_name: Dobberstein, B.
title: Identification of an H-2Kd gene using a specific cDNA probe
ispublished: pub
subjects: ddc-570
divisions: i-706000
keywords: histocompatibility genes , hybrid-selected translation , gene mapping , 3' non-coding region, Southern blot analysis
abstract: A cDNA clone known to code for a mouse histocompatibility (class I) antigen was found to contain a sequence specific for a subpopulation of H-2 genes. This unique sequence is located in the 3' non-coding region close to the stretch of poly(A) nucleotides. A subclone containing this fragment(pH-2d-5) has been used to select hybridizing mRNA. Translation of the mRNA in vitro shows that H-2Kd mRNA is selected. Southern blot analysis of DNA from congenic recombinant mice show that at least one gene containing this sequence is located at the K locus (region) of the major histocompatibility complex. This gene contains a 3.7-kb Bgml and a 13-kb EcoRI restriction endonuclease fragment. This gene has been isolated from a genomic DNA library. 
abstract_translated_lang: eng
date: 1982
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008595
schriftenreihe_cluster_id: sr-2
schriftenreihe_order: 022
ppn_swb: 1373878738
own_urn: urn:nbn:de:bsz:16-opus-85956
language: eng
bibsort: XINJHIDENTIFICA1982
full_text_status: public
publication: The EMBO Joumal
volume: 1
number: 4
pagerange: 467-471
citation:   Xin, J.-H. ; Kvist, S. ; Dobberstein, B.  (1982) Identification of an H-2Kd gene using a specific cDNA probe.  The EMBO Joumal, 1 (4).  pp. 467-471.      
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8595/1/Xin_1982_EMBOJ.pdf