title: Studying Integrin-Mediated Cell Spreading Using a Biomimetic System
creator: Streicher, Pia
subject: ddc-530
subject: 530 Physics
description: A novel system mimicking the first steps of integrin-mediated cell spreading was developed and characterized,  reconstituting the transmembrane protein integrin alphaII beta3 into giant unilamellar vesicles (GUVs). The method consisted of a detergent-mediated reconstitution of solubilized integrins into small proteoliposomes (0.1 - 0.2 µm in size) followed by electroswelling of a partially dried film of the same proteoliposomes. The reconstitution process was validated by analyzing protein incorporation and biological activity. Biological activity of integrin in GUVs was furthermore checked by adhesion tests onto biofunctionalized surface. The adhesion dynamics was studied experimentally and the results compared to theoretical predictions. The adhesion started with the spontaneous formation of several small (~200 nm) domains of tight adhesion, which grew and eventually fuse to form a ring-shaped adhesive patch at the border of the contact zone. This ring grew toward the center until it formed a uniformly adhering zone. Three distinct regimes were identified: For the first regime, the single adhesion zones were predicted to grow with R ~ t^5/8, which agreed well with our experimental data. Ring closure in the third regime was predicted to proceed with Ri ~ -t, which was also found in experiments. 
date: 2008
type: Dissertation
type: info:eu-repo/semantics/doctoralThesis
type: NonPeerReviewed
format: application/pdf
identifier: https://archiv.ub.uni-heidelberg.de/volltextserverhttps://archiv.ub.uni-heidelberg.de/volltextserver/8894/1/attempt1.pdf
identifier: DOI:10.11588/heidok.00008894
identifier: urn:nbn:de:bsz:16-opus-88943
identifier:   Streicher, Pia  (2008) Studying Integrin-Mediated Cell Spreading Using a Biomimetic System.  [Dissertation]     
relation: https://archiv.ub.uni-heidelberg.de/volltextserver/8894/
rights: info:eu-repo/semantics/openAccess
rights: http://archiv.ub.uni-heidelberg.de/volltextserver/help/license_urhg.html
language: eng