eprintid: 8904
rev_number: 8
eprint_status: archive
userid: 1
dir: disk0/00/00/89/04
datestamp: 2008-12-09 14:49:28
lastmod: 2014-04-03 21:02:02
status_changed: 2012-08-14 15:27:09
type: doctoralThesis
metadata_visibility: show
creators_name: Markgraf, Daniel Frank
title: The Role of Rab GTPases in the Endolysosomal System of S.cerevisiae
title_de: Die Rolle von RabGTPasen im Endolysosomalen System von S.cerevisiae
ispublished: pub
subjects: 570
divisions: 721000
adv_faculty: af-14
keywords: RabGTPase , Tethering FaktorenVesicular transport , RabGTPase , Tethering factor
cterms_swd: Membrantransport
cterms_swd: Endosom
cterms_swd: Vakuole
abstract: The evolution of the eucaryotic cell was accompanied by the development of an elaborate endomembrane system. Transport between intracellular compartments is mediated by vesicular carriers that constantly bud from donor membranes and fuse with target compartments. RabGTPases together with tethering factors mediate first specific contact between membranes destined fusion. This study focuses on the interplay between the endosomal RabGTPase Vps21 and the endosomal tethering complex, CORVET. The CORVET subunit Vps8 is the direct effector subunit and mediates binding to the GTP-form of Vps21, whereas the subunit Vps3 binds the GDP-form of Vps21. Vps8 and Vps21 functionally interact to mediate late endosomal tethering events. Furthermore, Vps8 and Vps21, together with the two other CORVET subunits, Vps3 and Vps16 were shown to be the minimal molecular requirement for late endosomal tethering.The results presented here indicate that the sequential recruitment of Vps8 to Vps21-positive late endosomes initiates tethering and leads to further assembly of the CORVET complex that dictates successive fusion events.
abstract_translated_text: The evolution of the eucaryotic cell was accompanied by the development of an elaborate endomembrane system. Transport between intracellular compartments is mediated by vesicular carriers that constantly bud from donor membranes and fuse with target compartments. RabGTPases together with tethering factors mediate first specific contact between membranes destined fusion. This study focuses on the interplay between the endosomal RabGTPase Vps21 and the endosomal tethering complex, CORVET. The CORVET subunit Vps8 is the direct effector subunit and mediates binding to the GTP-form of Vps21, whereas the subunit Vps3 binds the GDP-form of Vps21. Vps8 and Vps21 functionally interact to mediate late endosomal tethering events. Furthermore, Vps8 and Vps21, together with the two other CORVET subunits, Vps3 and Vps16 were shown to be the minimal molecular requirement for late endosomal tethering.The results presented here indicate that the sequential recruitment of Vps8 to Vps21-positive late endosomes initiates tethering and leads to further assembly of the CORVET complex that dictates successive fusion events.
abstract_translated_lang: eng
date: 2008
date_type: published
id_scheme: DOI
id_number: 10.11588/heidok.00008904
ppn_swb: 591110962
own_urn: urn:nbn:de:bsz:16-opus-89049
date_accepted: 2008-12-08
advisor: HASH(0x564e1c5adbe0)
language: eng
bibsort: MARKGRAFDATHEROLEOFR2008
full_text_status: public
citation:   Markgraf, Daniel Frank  (2008) The Role of Rab GTPases in the Endolysosomal System of S.cerevisiae.  [Dissertation]     
document_url: https://archiv.ub.uni-heidelberg.de/volltextserver/8904/1/PhD_thesis_D.Markgraf.pdf