%0 Generic
%A Hövener, Jan-Bernd
%D 2008
%F heidok:8912
%K Spin Hyperpolarisierung , PASADENASpin Hyperpolarizierung , PASADENA
%R 10.11588/heidok.00008912
%T 13C spin hyperpolarization by PASADENA : Instrumentation, preparation of magnetic tracers, and NMR spectroscopy and imaging in vivo
%U https://archiv.ub.uni-heidelberg.de/volltextserver/8912/
%X Techniques for the enhancement of the nuclear spin polarization (hyperpolarization) have demonstrated ~10 5 fold amplification of 13C NMR signal: new applications like imaging of metabolic processes in real-time and in vivo are in reach. PASADENA is a unique technique reaching high nuclear polarization (P) within seconds in liquid state: spin order of parahydrogen is transferred to a third nucleus by an r.f. spin-order-transfer (SOT) sequence. In this work, a semi-automated PASADENA polarizer for hyperpolarization of biomolecules in aqueous solution was constructed: P ~ 0.1 on 13C was demonstrated (a ~10 5 fold 13C signal enhancement at B0 = 1 T and T = 293 K). A simulation of the spin dynamics of the PASADENA experiment was developed to calculate the parameters for the SOT sequence and to predict the hyperpolarization yield. New compounds were introduced: the metabolic tracer 1-13C, 2,3-D2 succinate (Suc) and the functional agent 2,2,3,3-D4 tetraflouropropyl 1-13C, 2,3,3-D3 propionate (TFPP) were hyperpolarized (P ~ 0.1). The stability of the hyperpolarization was investigated in experiment and simulations. The lifetimes of the 13C hyperpolarization of Suc, TFPP and 2,2,3,3-hydroxyethyl 1-13C 2,3,3-D3 propionate (HEP) were determined in dependence of molecular deuteration, solvent deuteration, pH and B0: the maximal T1 = (59.7 ± 3.2) s of Suc prolongs the window for in vivo detection significantly. Strong enhancement of 13C NMR signal was observed in vivo after injection of hyperpolarized Suc and HEP in animals and cell cultures, demonstrating the potential biologically relevant molecules hyperpolarized by PASADENA in biomedical research.