TY  - GEN
Y1  - 2008///
KW  - Transkriptionsregulation 
KW  -  TBP 
KW  -  maternale RNA degradation 
KW  -  Promoterarchitekturtranscriptional regulation 
KW  -  TBP 
KW  -  maternal RNA degradation 
KW  -  promoter architecture
TI  - Large scale- and functional analysis for the requirement of TBP-function in early zebrafish development 
ID  - heidok8920
N2  - The differential expression of protein coding genes in specific cell types and during development requires the interaction of transcription factors with regulatory sequences in the proximal promoter to generate diverse expression patterns. In this thesis I address the differential regulatory function of TBP and the core promoter architecture facilitating this differential response in the complexity of the vertebrate organism by exploiting the experimental advantages of the zebrafish embryo model system. The work presented here demonstrates that only a proportion of genes require TBP-function in early zebrafish development and that TBP has a specific role in the clearance of maternal RNAs that includes the miR-430 pathway. These results indicate that TBP plays a major role in the transition from a transcriptionally inactive state to a transcriptionally active phase of the zebrafish embryo and has distinct functions in regulating gene expression during development. Furthermore, the bioinformatic characterisation of promoters regulated by TBP, as well as the functional analysis of the notail promoter, indicate that the TATA box, the core promoter motif TBP binds to, is not the defining feature of TBP-dependent transcription initiation mechanisms.
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/8920/
AV  - public
A1  - Ferg, Marco
ER  -