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primary topic: Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping

Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping
type: Article
abstract: Reactive oxygen species (ROS) have been reported to be locally produced after stimulation of various transmembrane receptors, including those for cytokines, growth factors and hormones. Transient ROS generation leads to the reversible oxidation of redox-sensitive proteins, which may either result in their transient activation or inactivation. Even though the role of ROS as a signal transducer is widely accepted, little is known about the identity of redox-sensitive proteins and the reduction mechanism by which they are re-generated. Thioredoxin-1 (Trx1) is an oxidoreductase known to influence a variety of cellular processes including proliferation, apoptosis and gene expression. In higher plants and cyanobacteria different proteomic approaches allowed for the identification of various thioredoxin target proteins. However, no comprehensive study of Trx1 interactions in the cytosol of mammalian cells has been performed so far. In this thesis, mechanism-based kinetic trapping was applied to identify proteins forming mixed disulfide intermediates with Trx1 in the cytosol of a human T cell leukemia line. Most previously established target proteins of Trx1 could be identified, including ribonucleotide reductase, peroxiredoxins and annexin-2, thus confirming the validity of the approach. Interestingly, a substantial number of newly identified proteins currently lacks functional annotation, suggesting that several thioredoxin-regulated pathways still await initial characterization. Of those newly identified proteins with known function, a significant portion is associated with cell cycle control and regulation of apoptosis, while others participate in various signal transduction pathways, cytoskeleton and membrane dynamics, metabolism and transcriptional control. These results strongly support the long-held belief that Trx1 not only plays a role in ROS scavenging and reductive metabolism but is also extensively involved in interactions with key regulatory proteins associate…
date: 2008
status: published
author list: authors
type: EPrint
type: DoctoralThesisEPrint
is part of: repository
has document: Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (PDF)
has document: Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (Other)
subject: 570 Biowissenschaften, Biologie

Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (PDF)
type: Document
has file: Lars_Weingarten_Phd_Thesis.pdf
has part: Lars_Weingarten_Phd_Thesis.pdf

Lars Weingarten
type: Person
given name: Lars
family name: Weingarten

Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (Other)
type: Document
is version of: Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (PDF)
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Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (Other)
type: Document
is version of: Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (PDF)
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Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (Other)
type: Document
is version of: Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (PDF)
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Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (Other)
type: Document
is version of: Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (PDF)
is volatile version of: Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (PDF)
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Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (Other)
type: Document
is version of: Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (PDF)
is volatile version of: Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping (PDF)
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primary topic: Identification of novel cytosolic thioredoxin-1 target proteins in mammalian cells by mechanism-based kinetic trapping

570 Biowissenschaften, Biologie
type: Concept
preferred label: 570 Life sciences @en