<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Cellular Separation Forces Studied by Force Spectroscopy and Microplate Manipulation"^^ . "During adaptive immune response, peptides from foreign microorganisms are processed and displayed on the surface of Antigen Presenting Cells (APCs) within the cleft of major histocompatibility complexes (pMHC), which are recognized by T cells that patrol the body. In recent years, a large spatially organized molecular cluster called immune synapse (IS) has been identifed, which forms at the T cell/APC interface. Little is known about the biophysics of these cell conjugates and it is generally believed that cell adhesion molecules are activated on the T cell surface after recognition, which presumably leads to higher adhesion forces between the two cells. Thus, the goal of this thesis was to measure the change of total adhesion forces between T cells and APCs when peptide was present compared to the case when peptide was absent. In this work, we report the first measurement of separation forces between T cells and APCs employing a cell stretcher device and Atomic Force Microscopy (AFM). We analyze the kinetics of T cells that interact with APCs presenting a foreign peptide by their MHC (pulsed), and APCs carrying no peptide (unpulsed) for contact times of 0 to 60 minutes. Unlike pulsed cells, unpulsed APCs did not show the formation of immune synapses. Forces are comparable for short time points but start to deviate after 15 min to reach a maximum after 30 min in the pulsed case. We were able to correlate single rupture events in AFM spectra to protein unbinding theories and, hence, gained a deeper insight into the molecular basis for the increase in adhesion forces. Thus, this work provides an important contribution to the physical understanding of cell-cell adhesion. "^^ . "2008" . . . . . . . . "Babak"^^ . "Hadji Hosseini"^^ . "Babak Hadji Hosseini"^^ . . . . . . "Cellular Separation Forces Studied by Force Spectroscopy and Microplate Manipulation (PDF)"^^ . . . "dissertationA.pdf"^^ . . . "Cellular Separation Forces Studied by Force Spectroscopy and Microplate Manipulation (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Cellular Separation Forces Studied by Force Spectroscopy and Microplate Manipulation (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Cellular Separation Forces Studied by Force Spectroscopy and Microplate Manipulation (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Cellular Separation Forces Studied by Force Spectroscopy and Microplate Manipulation (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Cellular Separation Forces Studied by Force Spectroscopy and Microplate Manipulation (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #9147 \n\nCellular Separation Forces Studied by Force Spectroscopy and Microplate Manipulation\n\n" . "text/html" . . . "530 Physik"@de . "530 Physics"@en . .