<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "3D fluorescence microscopy with isotropic resolution on the nanoscale"^^ . "The resolution of any linear imaging system is given by its point-spread-function (PSF) quantifying the blur of an object point in the image. The sharper the PSF, the better is the resolution. In standard fluorescence microscopy, however, diffraction dictates a PSF with a cigar-shaped main maximum, called the focal spot which extends over at least half the wavelength of light (L = 400-800 nm) in the focal plane and > L along the optic axis (z). While concepts have evolved to sharpen the focal spot both laterally and axially, none of them has reached their ultimate goal: a spherical spot that can be arbitrarily downscaled in size. Herein, I introduce such a fluorescence microscope and demonstrate the creation of spherical focal spots of 40-45 nm (~ L/16) diameter that is pushed down to 21-30 nm (~ L/30) under suitable conditions. Fully relying on focused light, this lens-based fluorescence nanoscope unravels the interior of cells noninvasively, uniquely dissecting their sub-L sized organelles. Further fields of application open up, such as the characterization of novel nanomaterials. "^^ . "2008" . . . . . . . . "Roman"^^ . "Schmidt"^^ . "Roman Schmidt"^^ . . . . . . "3D fluorescence microscopy with isotropic resolution on the nanoscale (PDF)"^^ . . . "Thesis_Roman_Schmidt.pdf"^^ . . . "3D fluorescence microscopy with isotropic resolution on the nanoscale (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "3D fluorescence microscopy with isotropic resolution on the nanoscale (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "3D fluorescence microscopy with isotropic resolution on the nanoscale (Other)"^^ . . . . . . "preview.jpg"^^ . . . "3D fluorescence microscopy with isotropic resolution on the nanoscale (Other)"^^ . . . . . . "medium.jpg"^^ . . . "3D fluorescence microscopy with isotropic resolution on the nanoscale (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #9396 \n\n3D fluorescence microscopy with isotropic resolution on the nanoscale\n\n" . "text/html" . . . "530 Physik"@de . "530 Physics"@en . .