<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "A Genetic Approach towards Identification of Factors Affecting Quinine Accumulation in P. falciparum"^^ . "Although PfCRT has been implicated as the main factor affecting chloroqune accumulation and resistance in Plasmodium falciparum, quinine accumulation and resistance have long been suspected to be affected by more than one genetic factor. Prior studies demonstrated that reduced quinine accumulation did not necessarily cause increased quinine resistance (Sanchez et al., 2008b), indicating that resistance and accumulation did not share all factors affecting them. In this study, the initial accumulation levels of radio-labelled quinine in the progeny set of the HB3 x Dd2 genetic cross were investigated and quantitative trait loci (QTL) analysis was performed based on these data. By measuring the initial quinine accumulation, a read-out defined by processes contributing to early quinine accumulation, such as facilitating, transporting or partitioning processes, would be obtained, thereby isolating these processes from the later events that cause cell death, such as inhibition of haem mineralisation or of some enzymatic function. The IC90 data of the same progeny set (Ferdig et al., 2004) were reanalysed and comparing the QTL analysis data obtained from quinine initial accumulation and IC90 levels allowed isolation of factors affecting only cell deaths from those that also affected drug transport. A novel locus in chromosome 7, B5M12, seems to have a major effect to both quinine accumulation and IC90. The locus acts synergistically with pfcrt. Two loci in chromosome 13, VAPA and C13M73, showed major effect on quinine IC90, but had no effect to the accumulation. Despite the prediction that the putative NA+/H+ exchanger (PfNHE) is involved in quinine resistance, the result revealed that the putative gene was not located within these two peaks. The Dd2 alleles of VAPA and C13M73 were associated with higher quinine IC90. The secondary scan showed the involvement of pfmdr1 in both quinine accumulation and IC90. Interestingly, the Dd2-type pfmdr1 contributes to an increase in the initial quinine accumulation, and, correspondingly, to an increase in the sensitivity to quinine (the IC90 being decreased)."^^ . "2009" . . . . . . . . "Astutiati"^^ . "Nurhasanah"^^ . "Astutiati Nurhasanah"^^ . . . . . . "A Genetic Approach towards Identification of Factors Affecting Quinine Accumulation in P. falciparum (PDF)"^^ . . . "Thesisku_revised.pdf"^^ . . . "A Genetic Approach towards Identification of Factors Affecting Quinine Accumulation in P. falciparum (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "A Genetic Approach towards Identification of Factors Affecting Quinine Accumulation in P. falciparum (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "A Genetic Approach towards Identification of Factors Affecting Quinine Accumulation in P. falciparum (Other)"^^ . . . . . . "preview.jpg"^^ . . . "A Genetic Approach towards Identification of Factors Affecting Quinine Accumulation in P. falciparum (Other)"^^ . . . . . . "medium.jpg"^^ . . . "A Genetic Approach towards Identification of Factors Affecting Quinine Accumulation in P. falciparum (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #9484 \n\nA Genetic Approach towards Identification of Factors Affecting Quinine Accumulation in P. falciparum\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .