<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Developing a Zebrafish Model for Muscle Regeneration"^^ . "A better understanding of muscle regeneration would allow us to devise therapies that are more effective for patients suffering from myodegenerative diseases such as muscular dystrophies. Animal models facilitate the understanding of muscle regeneration, but so far, only rodents and chicken have been suitably exploited in this regard. In the present study, I adopted three different approaches to establish a model of muscle regeneration in zebrafish. Firstly, an ENU mutagenesis screen was performed for mutants with defective muscle maintenance that might result from faulty regeneration. I identified and characterized one mutant (gum) showing progressive loss of motility and myofibrillar organization. Characterization of gum mutants revealed multiple defects in muscle, neuronal and neural crest derived tissues. Secondly, using an inhibitor of acetycholinesterase (GAL), I established a chemically inducible model of myopathy in zebrafish. Removal of GAL allowed the muscles to regenerate and restored their normal function. Based on electron microscopy and immunohistochemistry, the zebrafish putative muscle stem cells (satellite cells) were identified. Pax7, a key marker for satellite cells in all vertebrates, labels a layer of cells, the dermomyotome, on the surface of zebrafish somites from 24 hpf onwards. These cells give rise initially, to the fast muscle fibers and later to the satellite cells. In this study, it was observed that the Pax7+ve dermomyotome cells show increased proliferation and migration into deeper myotome upon muscle damage. Thirdly, unbiased genome wide transcriptional profiling of GAL treated zebrafish larvae showed numerous genes upregulated in response to the myopathy. Comparison of these genes to mouse models of muscle regeneration showed a significant (about 25%) overlap. Expression analysis of some of the genes (cmya1, zgc:100919) indicates that they might have a role in satellite cells biology."^^ . "2009" . . . . . . . . "Sandeep"^^ . "Paul"^^ . "Sandeep Paul"^^ . . . . . . "Developing a Zebrafish Model for Muscle Regeneration (PDF)"^^ . . . "Sandeep_Thesis.pdf"^^ . . . "Developing a Zebrafish Model for Muscle Regeneration (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "Developing a Zebrafish Model for Muscle Regeneration (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Developing a Zebrafish Model for Muscle Regeneration (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Developing a Zebrafish Model for Muscle Regeneration (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Developing a Zebrafish Model for Muscle Regeneration (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #9697 \n\nDeveloping a Zebrafish Model for Muscle Regeneration\n\n" . "text/html" . . . "570 Biowissenschaften, Biologie"@de . "570 Life sciences"@en . .