TY  - GEN
TI  - Adaptive elements for STED microscopy
N2  - Modern fluorescence far-field microscopes are not resolution limited by diffraction, anymore. This property increases the impact of these microscopes for cell-biological imaging. This thesis covers methods, that considerably improve the quality of ensemble scanning microscopes, a subclass of modern fluorescence microscopes. The influence of the PSF is optimized with a spatial light modulator (SLM) in case of stimulated emission depletion (STED) microscopy to increase the resolution and the signal noise ratio of the microscope. Together with a novel error correction routine the SLM provides unrivaled flexibility and quality for the generated STED-PSF. Furthermore, a new adaptive beam scanning pattern is proposed, which shortens image acquisition time and simultaneously reduces illumination load of the sample with else unimpaired image quality. This concept is extended by another novel locally adaptive illumination method, which reduces photo bleaching, dark state transitions and phototoxic effects several factors. These effects are further decreased with better resolution. A combination of these adaptive methods enables unprecedented scan times for imaging of fast dynamic processes and enables 3D STED images previously frustrated by photo bleaching.
A1  - Engler, Andreas
AV  - public
UR  - https://archiv.ub.uni-heidelberg.de/volltextserver/9941/
KW  - BleichreduzierungLivecell Imaging 
KW  -  Adaptive scanning 
KW  -  STED 
KW  -  Spatial light modulator 
KW  -  bleach reduction
ID  - heidok9941
Y1  - 2009///
ER  -