In: Epigenetics & Chromatin, 9 (2016), Nr. 57. pp. 1-20. ISSN 1756-8935
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Abstract
Background: Genome organization into subchromosomal topologically associating domains (TADs) is linked to cell-type-specific gene expression programs. However, dynamic properties of such domains remain elusive, and it is unclear how domain plasticity modulates genomic accessibility for soluble factors. Results: Here, we combine and compare a high-resolution topology analysis of interacting chromatin loci with fluorescence correlation spectroscopy measurements of domain dynamics in single living cells. We identify topologically and dynamically independent chromatin domains of ~1 Mb in size that are best described by a loop-cluster polymer model. Hydrodynamic relaxation times and gyration radii of domains are larger for open (161 ± 15 ms, 297 ± 9 nm) than for dense chromatin (88 ± 7 ms, 243 ± 6 nm) and increase globally upon chromatin hyperacetylation or ATP depletion. Conclusions: Based on the domain structure and dynamics measurements, we propose a loop-cluster model for chromatin domains. It suggests that the regulation of chromatin accessibility for soluble factors displays a significantly stronger dependence on factor concentration than search processes within a static network.
Document type: | Article |
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Journal or Publication Title: | Epigenetics & Chromatin |
Volume: | 9 |
Number: | 57 |
Publisher: | BioMed Central |
Place of Publication: | London |
Date Deposited: | 04 Jan 2017 13:23 |
Date: | 2016 |
ISSN: | 1756-8935 |
Page Range: | pp. 1-20 |
Faculties / Institutes: | Service facilities > Bioquant Service facilities > CellNetworks Core Technology Platform Service facilities > German Cancer Research Center (DKFZ) Service facilities > European Molecular Biology Laboratory (EMBL) |
DDC-classification: | 610 Medical sciences Medicine |