In: Epigenetics & Chromatin, 9 (2016), Nr. 57. S. 1-20. ISSN 1756-8935

Vorschau

PDF, Englisch Download (2MB) | Lizenz: Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells von Wachsmuth, Malte ; Knoch, Tobias A. ; Rippe, Karsten steht unter einer Creative Commons Namensnennung 3.0 Deutschland

Abstract

Background: Genome organization into subchromosomal topologically associating domains (TADs) is linked to cell-type-specific gene expression programs. However, dynamic properties of such domains remain elusive, and it is unclear how domain plasticity modulates genomic accessibility for soluble factors. Results: Here, we combine and compare a high-resolution topology analysis of interacting chromatin loci with fluorescence correlation spectroscopy measurements of domain dynamics in single living cells. We identify topologically and dynamically independent chromatin domains of ~1 Mb in size that are best described by a loop-cluster polymer model. Hydrodynamic relaxation times and gyration radii of domains are larger for open (161 ± 15 ms, 297 ± 9 nm) than for dense chromatin (88 ± 7 ms, 243 ± 6 nm) and increase globally upon chromatin hyperacetylation or ATP depletion. Conclusions: Based on the domain structure and dynamics measurements, we propose a loop-cluster model for chromatin domains. It suggests that the regulation of chromatin accessibility for soluble factors displays a significantly stronger dependence on factor concentration than search processes within a static network.