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The importance of claudin-7 palmitoylation on membrane subdomain localization and metastasis-promoting activities

Heiler, Sarah ; Mu, Wei ; Zöller, Margot ; Thuma, Florian

In: Cell communication and signaling, 13 (2015), Nr. 29. pp. 1-17. ISSN 1478-811X

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Download (4MB) | Lizenz: Creative Commons LizenzvertragThe importance of claudin-7 palmitoylation on membrane subdomain localization and metastasis-promoting activities by Heiler, Sarah ; Mu, Wei ; Zöller, Margot ; Thuma, Florian underlies the terms of Creative Commons Attribution 3.0 Germany

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Abstract

Background: Claudin-7 (cld7), a tight junction (TJ) component, is also found basolaterally and in the cytoplasm. Basolaterally located cld7 is enriched in glycolipid-enriched membrane domains (GEM), where it associates with EpCAM (EpC). The conditions driving cld7 out of TJ into GEM, which is associated with a striking change in function, were not defined. Thus, we asked whether cld7 serines or palmitoylation affect cld7 location and protein, particularly EpCAM, associations. Results: HEK cells were transfected with EpCAM and wild type cld7 or cld7, where serine phopsphorylation or the palmitoylation sites (AA184, AA186) (cld7mPalm) were mutated. Exchange of individual serine phosphorylation sites did not significantly affect the GEM localization and the EpCAM association. Instead, cld7mPalm was poorly recruited into GEM. This has consequences on migration and invasiveness as palmitoylated cld7 facilitates integrin and EpCAM recruitment, associates with cytoskeletal linker proteins and cooperates with MMP14, CD147 and TACE, which support motility, matrix degradation and EpCAM cleavage. On the other hand, only cld7mPalm associates with TJ proteins. Conclusion: Cld7 palmitoylation prohibits TJ integration and fosters GEM recruitment. Via associated molecules, palmitoylated cld7 supports motility and invasion.

Item Type: Article
Journal or Publication Title: Cell communication and signaling
Volume: 13
Number: 29
Publisher: Biomed Central
Place of Publication: London
Date Deposited: 25 Nov 2015 10:12
Date: 2015
ISSN: 1478-811X
Page Range: pp. 1-17
Faculties / Institutes: Service facilities > German Cancer Research Center (DKFZ)
Medizinische Fakultät Heidelberg > Chirurgische Universitätsklinik
Subjects: 610 Medical sciences Medicine
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