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Antibodies to the inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) in cerebellar ataxia

Jarius, Sven ; Scharf, Madeleine ; Begemann, Nora ; Stöcker, Winfried ; Probst, Christian ; Serysheva, Irina I. ; Nagel, Sigrun ; Graus, Francesc ; Psimaras, Dimitri ; Wildemann, Brigitte ; Komorowski, Lars

In: Journal of neuroinflammation: JNI, 11 (2014), Nr. 206. pp. 1-12. ISSN 1742-2094

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Download (3MB) | Lizenz: Creative Commons LizenzvertragAntibodies to the inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) in cerebellar ataxia by Jarius, Sven ; Scharf, Madeleine ; Begemann, Nora ; Stöcker, Winfried ; Probst, Christian ; Serysheva, Irina I. ; Nagel, Sigrun ; Graus, Francesc ; Psimaras, Dimitri ; Wildemann, Brigitte ; Komorowski, Lars underlies the terms of Creative Commons Attribution 3.0 Germany

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Abstract

We report on a serum autoantibody associated with cerebellar ataxia. Immunohistochemical studies of sera from four patients referred for autoantibody testing revealed binding of high-titer (up to 1:5,000) IgG antibodies, mainly IgG1, to the molecular layer, Purkinje cell layer, and white matter on mouse, rat, porcine, and monkey cerebellum sections. The antibody bound to PC somata, dendrites, and axons, resulting in a binding pattern similar to that reported for anti-Ca/anti-ARHGAP26, but did not react with recombinant ARHGAP26. Extensive control studies were performed to rule out a broad panel of previously described paraneoplastic and non-paraneoplastic anti-neural autoantibodies. The characteristic binding pattern as well as double staining experiments suggested inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) as the target antigen. Verification of the antigen included specific neutralization of the tissue reaction following preadsorption with ITPR1 (but not ARHGAP26) and a dot-blot assay with purified ITPR1 protein. By contrast, anti-ARHGAP26-positive sera did not bind to ITPR1. In a parallel approach, a combination of histoimmunoprecipitation and mass spectrometry also identified ITPR1 as the target antigen. Finally, a recombinant cell-based immunofluorescence assay using HEK293 cells expressing ITPR1 and ARHGAP26, respectively, confirmed the identification of ITPR1. Mutations of ITPR1 have previously been implicated in spinocerebellar ataxia with and without cognitive decline. Our findings suggest a role of autoimmunity against ITPR1 in the pathogenesis of autoimmune cerebellitis and extend the panel of diagnostic markers for this disease.

Item Type: Article
Journal or Publication Title: Journal of neuroinflammation: JNI
Volume: 11
Number: 206
Publisher: BioMed Central
Place of Publication: London
Date Deposited: 25 Nov 2015 10:39
Date: 2014
ISSN: 1742-2094
Page Range: pp. 1-12
Faculties / Institutes: Medizinische Fakultät Heidelberg > Neurologische Universitätsklinik
Subjects: 570 Life sciences
610 Medical sciences Medicine
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