Direkt zum Inhalt
  1. Publizieren |
  2. Suche |
  3. Browsen |
  4. Neuzugänge rss |
  5. Open Access |
  6. Rechtsfragen |
  7. EnglishCookie löschen - von nun an wird die Spracheinstellung Ihres Browsers verwendet.

Blood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured

Kahn, Nicolas ; Riedlinger, Julia ; Roeßler, Markus ; Rabe, Christina ; Lindner, Michael ; Koch, Ina ; Schott-Hildebrand, Sabine ; Herth, Felix J. ; Schneider, Marc A. ; Meister, Michael ; Muley, Thomas R.

In: Clinical proteomics, 12 (2015), Nr. 19. S. 1-7. ISSN 1559-0275

[thumbnail of 12014_2015_Article_9093.pdf]
Vorschau
PDF, Englisch
Download (1MB) | Lizenz: Creative Commons LizenzvertragBlood-sampling collection prior to surgery may have a significant influence upon biomarker concentrations measured von Kahn, Nicolas ; Riedlinger, Julia ; Roeßler, Markus ; Rabe, Christina ; Lindner, Michael ; Koch, Ina ; Schott-Hildebrand, Sabine ; Herth, Felix J. ; Schneider, Marc A. ; Meister, Michael ; Muley, Thomas R. steht unter einer Creative Commons Namensnennung 3.0 Deutschland

Zitieren von Dokumenten: Bitte verwenden Sie für Zitate nicht die URL in der Adresszeile Ihres Webbrowsers, sondern entweder die angegebene DOI, URN oder die persistente URL, deren langfristige Verfügbarkeit wir garantieren. [mehr ...]

Abstract

Abstract Background Biomarkers can be subtle tools to aid the diagnosis, prognosis and monitoring of therapy and disease progression. The validation of biomarkers is a cumbersome process involving many steps. Serum samples from lung cancer patients were collected in the framework of a larger study for evaluation of biomarkers for early detection of lung cancer. The analysis of biomarker levels measured revealed a noticeable difference in certain biomarker values that exhibited a dependence of the time point and setting of the sampling. Biomarker concentrations differed significantly if taken before or after the induction of anesthesia and if sampled via venipuncture or arterial catheter. Methods To investigate this observation, blood samples from 13 patients were drawn 1–2 days prior to surgery (T1), on the same day by venipuncture (T2) and after induction of anesthesia via arterial catheter (T3). The biomarkers Squamous Cell Carcinoma antigen (CanAG SCC EIA, Fujirebio Diagnostics, Malvern, USA), Carcinoembrionic Antigen (CEA), and CYFRA 21-1 (Roche Diagnostics GmbH, Mannheim, Germany) were analyzed. Results SCC showed a very strong effect in relation to the sampling time and procedure. While the first two points in time (T1; T2) were highly comparable (median fold-change: 0.84; p = 0.7354; correlation ρ = 0.883), patients showed a significant increase (median fold-change: 4.96; p = 0.0017; correlation ρ = -0.036) in concentration when comparing T1 with the sample time subsequent to anesthesia induction (T3). A much weaker increase was found for CYFRA 21-1 at T3 (median fold-change: 1.40; p = 0.0479). The concentration of CEA showed a very small, but systematic decrease (median fold-change: 0.72; p = 0.0039). Conclusions In this study we show the unexpectedly marked influence of blood withdrawal timing (before vs. after anesthesia) and procedure (venous versus arterial vessel puncture) has on the concentration of the protein biomarker SCC and to a less extent upon CYFRA21-1. The potential causes for these effects remain to be elucidated in subsequent studies, however these findings highlight the importance of a standardized, controlled blood collection protocol for biomarker detection.

Dokumententyp: Artikel
Titel der Zeitschrift: Clinical proteomics
Band: 12
Nummer: 19
Verlag: BioMed Central
Ort der Veröffentlichung: London
Erstellungsdatum: 08 Jan. 2016 13:06
Erscheinungsjahr: 2015
ISSN: 1559-0275
Seitenbereich: S. 1-7
Institute/Einrichtungen: Medizinische Fakultät Heidelberg und Uniklinikum > Thoraxklinik Heidelberg gGmbH
DDC-Sachgruppe: 610 Medizin
Leitlinien | Häufige Fragen | Kontakt | Impressum |
OA-LogoDINI-Zertifikat 2013Logo der Open-Archives-Initiative