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The methylome of the marbled crayfish links gene body methylation to stable expression of poorly accessible genes

Gatzmann, Fanny ; Falckenhayn, Cassandra ; Gutekunst, Julian ; Hanna, Katharina ; Raddatz, Günter ; Carneiro, Vitor Coutinho ; Lyko, Frank

In: Epigenetics & Chromatin, 11 (2018), Nr. 57. pp. 1-12. ISSN 1756-8935

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Download (3MB) | Lizenz: Creative Commons LizenzvertragThe methylome of the marbled crayfish links gene body methylation to stable expression of poorly accessible genes by Gatzmann, Fanny ; Falckenhayn, Cassandra ; Gutekunst, Julian ; Hanna, Katharina ; Raddatz, Günter ; Carneiro, Vitor Coutinho ; Lyko, Frank underlies the terms of Creative Commons Attribution 3.0 Germany

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Abstract

Background: The parthenogenetic marbled crayfish (Procambarus virginalis) is a novel species that has rapidly invaded and colonized various different habitats. Adaptation to different environments appears to be independent of the selection of genetic variants, but epigenetic programming of the marbled crayfish genome remains to be understood.

Results: Here, we provide a comprehensive analysis of DNA methylation in marbled crayfish. Whole-genome bisulfite sequencing of multiple replicates and different tissues revealed a methylation pattern that is characterized by gene body methylation of housekeeping genes. Interestingly, this pattern was largely tissue invariant, suggesting a function that is unrelated to cell fate specification. Indeed, integrative analysis of DNA methylation, chromatin accessibility and mRNA expression patterns revealed that gene body methylation correlated with limited chromatin accessibility and stable gene expression, while low-methylated genes often resided in chromatin with higher accessibility and showed increased expression variation. Interestingly, marbled crayfish also showed reduced gene body methylation and higher gene expression variability when compared with their noninvasive mother species, Procambarus fallax.

Conclusions: Our results provide novel insights into invertebrate gene body methylation and its potential role in adaptive gene regulation.

Item Type: Article
Journal or Publication Title: Epigenetics & Chromatin
Volume: 11
Number: 57
Publisher: BioMed Central
Place of Publication: London
Date Deposited: 15 Oct 2018 09:01
Date: 2018
ISSN: 1756-8935
Page Range: pp. 1-12
Faculties / Institutes: Service facilities > German Cancer Research Center (DKFZ)
Subjects: 610 Medical sciences Medicine
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