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Rpv3–1 mediated resistance to grapevine downy mildew is associated with specific host transcriptional responses and the accumulation of stilbenes

Eisenmann, Birgit ; Czemmel, Stefan ; Ziegler, Tobias ; Buchholz, Günther ; Kortekamp, Andreas ; Trapp, Oliver ; Rausch, Thomas ; Dry, Ian ; Bogs, Jochen

In: BMC Plant Biology, 19 (2019), Nr. 343. pp. 1-17. ISSN 1471-2229

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Download (2MB) | Lizenz: Creative Commons LizenzvertragRpv3–1 mediated resistance to grapevine downy mildew is associated with specific host transcriptional responses and the accumulation of stilbenes by Eisenmann, Birgit ; Czemmel, Stefan ; Ziegler, Tobias ; Buchholz, Günther ; Kortekamp, Andreas ; Trapp, Oliver ; Rausch, Thomas ; Dry, Ian ; Bogs, Jochen underlies the terms of Creative Commons Attribution 4.0

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Abstract

Background: European grapevine cultivars (Vitis vinifera spp.) are highly susceptible to the downy mildew pathogen Plasmopara viticola. Breeding of resistant V. vinifera cultivars is a promising strategy to reduce the impact of disease management. Most cultivars that have been bred for resistance to downy mildew, rely on resistance mediated by the Rpv3 (Resistance to P. viticola) locus. However, despite the extensive use of this locus, little is known about the mechanism of Rpv3-mediated resistance.

Results: In this study, Rpv3-mediated defense responses were investigated in Rpv3+ and Rpv3ˉ grapevine cultivars following inoculation with two distinct P. viticola isolates avrRpv3+ and avrRpv3ˉ, with the latter being able to overcome Rpv3 resistance. Based on comparative microscopic, metabolomic and transcriptomic analyses, our results show that the Rpv3–1-mediated resistance is associated with a defense mechanism that triggers synthesis of fungi-toxic stilbenes and programmed cell death (PCD), resulting in reduced but not suppressed pathogen growth and development. Functional annotation of the encoded protein sequence of genes significantly upregulated during the Rpv3–1-mediated defense response revealed putative roles in pathogen recognition, signal transduction and defense responses.

Conclusion: This study used histochemical, transcriptomic and metabolomic analyses of Rpv3+ and susceptible cultivars inoculated with avirulent and virulent P. viticola isolates to investigate mechanism underlying the Rpv3–1-mediated resistance response. We demonstrated a strong correlation between the expressions of stilbene biosynthesis related genes, the accumulation of fungi-toxic stilbenes, pathogen growth inhibition and PCD.

Item Type: Article
Journal or Publication Title: BMC Plant Biology
Volume: 19
Number: 343
Publisher: BioMed Central
Place of Publication: London
Date Deposited: 21 Aug 2019 10:24
Date: 2019
ISSN: 1471-2229
Page Range: pp. 1-17
Faculties / Institutes: Service facilities > Centre for Organismal Studies Heidelberg (COS)
Subjects: 610 Medical sciences Medicine
Uncontrolled Keywords: Disease resistance, Downy mildew, Grapevine, Plasmopara viticola, Stilbenes, Vitis vinifera, Metabolomics, Rpv3
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