In: Transfusion Medicine and Hemotherapy, 40 (2013), Nr. 5. pp. 326-335. ISSN 1660-3796 (Druck-Ausg.), 1660-3818 (Online-Ausg.)
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Abstract
Mesenchymal stromal cells (MSC) emerged as highly attractive in cell-based regenerative medicine. Initially thought to provide cells capable of differentiation towards mesenchymal cell types (osteoblasts, chondrocytes, adipocytes etc.), by and by potent immunoregulatory and pro-regenerative activities have been discovered, broadening the field of potential applications from bone and cartilage regeneration to wound healing and treatment of autoimmune diseases. Due to the limited frequency in most tissue sources, ex vivo expansion of MSC is required compliant with good manufacturing practice (GMP) guidelines to yield clinically relevant cell doses. Though, still most manufacturing protocols use fetal bovine serum (FBS) as cell culture supplement to isolate and to expand MSC. However, the high lot-to-lot variability as well as risk of contamination and immunization call for xenogenic-free culture conditions. In terms of standardization, chemically defined media appear as the ultimate achievement. Since these media need to maintain all key cellular and therapy-relevant features of MSC, the development of chemically defined media is still – albeit highly investigated – only in its beginning. The current alternatives to FBS rely on human blood-derived components: plasma, serum, umbilical cord blood serum, and platelet derivatives like platelet lysate. Focusing on quality aspects, the latter will be addressed within this review.
Document type: | Article |
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Journal or Publication Title: | Transfusion Medicine and Hemotherapy |
Volume: | 40 |
Number: | 5 |
Publisher: | Karger |
Place of Publication: | Basel ; Freiburg |
Date Deposited: | 17 Oct 2019 12:41 |
Date: | 2013 |
ISSN: | 1660-3796 (Druck-Ausg.), 1660-3818 (Online-Ausg.) |
Page Range: | pp. 326-335 |
Faculties / Institutes: | Medizinische Fakultät Mannheim > Institut für Transfusionsmedizin und Immunologie |
DDC-classification: | 610 Medical sciences Medicine |
Uncontrolled Keywords: | platelet lysate , platelet releasate , mesenchymal stromal cells , fetal bovine serum , good manufacturing practice |
Additional Information: | Dieser Beitrag ist aufgrund einer (DFG-geförderten) Allianz bzw. Nationallizenz frei zugänglich. This publication is freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively. |