Supplemental Materials: Novel Isotypic g/z-Subunits Reveal Three Coatomer Complexes in Mammals

Wegmann, Dominik and Hess, Pablo and Baier, Carola and Wieland, Felix T. and Reinhard, Constanze

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Abstract

Fig. A 1 shows a Western blot analyzing the specificity of the antibodies against g- and z-isotypes. These antibodies were probed in different mammalian species (Fig. A 2). Coatomer was labeled with [35S]-methionine for different time periods, and the incorporation of [35S]-methionine as well as the corresponding stoichiometries of the coatomer subunits are shown in Fig. A 3. Characterization of anti-coatomer antibody 883 is depicted in Fig. A 4.

Item Type:	Other
Date:	2003
Faculties / Institutes:	Service facilities > Heidelberg University Biochemistry Center
Subjects:	570 Life sciences
Controlled Keywords:	Golgi, COPI vesicles, coatomer isotypes, secretory pathway, g2-COP and z2-COP