Fig. A 1 shows a Western blot analyzing the specificity of the antibodies against g- and z-isotypes. These antibodies were probed in different mammalian species (Fig. A 2). Coatomer was labeled with [35S]-methionine for different time periods, and the incorporation of [35S]-methionine as well as the corresponding stoichiometries of the coatomer subunits are shown in Fig. A 3. Characterization of anti-coatomer antibody 883 is depicted in Fig. A 4.
|Faculties / Institutes:||Service facilities > Heidelberg University Biochemistry Center|
|Subjects:||570 Life sciences|
|Controlled Keywords:||Golgi, COPI vesicles, coatomer isotypes, secretory pathway, g2-COP and z2-COP|