Wegmann, Dominik ; Hess, Pablo ; Baier, Carola ; Wieland, Felix T. ; Reinhard, Constanze
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Abstract
Fig. A 1 shows a Western blot analyzing the specificity of the antibodies against g- and z-isotypes. These antibodies were probed in different mammalian species (Fig. A 2). Coatomer was labeled with [35S]-methionine for different time periods, and the incorporation of [35S]-methionine as well as the corresponding stoichiometries of the coatomer subunits are shown in Fig. A 3. Characterization of anti-coatomer antibody 883 is depicted in Fig. A 4.
Document type: | Other |
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Date Deposited: | 06 Jan 2003 13:55 |
Date: | 2003 |
Faculties / Institutes: | Service facilities > Heidelberg University Biochemistry Center |
DDC-classification: | 570 Life sciences |
Controlled Keywords: | Golgi, COPI vesicles, coatomer isotypes, secretory pathway, g2-COP and z2-COP |