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In vitro synthesis and processing of a putative precursor for the small subunit of ribulose- 1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii

Dobberstein, Bernhard and Blobel, Günter and Chua, Nam-Hai

In: Proc. Natl. Acad. Sci. USA, 74 (1977), Nr. 3. pp. 1082-1085

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Abstract

Translation of polyadenylated mRNA of Chlamydomonas reinhardtii in a cell-free wheat germ system resulted in the synthesis of numerous discrete polypeptides. Among them was a species with molecular weight 20,000 that was immunoprecipitated specifically by antibodies raised against the authentic small subunit (16,500 daltons) of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase(dimerizing), EC 4.1.1.39]. Since the immunoprecipitated polypeptide has a larger molecular weight by approximately 3500 than the small subunit (S) it was identified as a putative biosynthetic precursor (pS). Post-translational conversion of pS by a specific endoprotease yielded two detectable products: one apparently identical in size to S and the other, a small peptide, presumably representing the remainder of pS. The endoprotease requires sulfhydryl groups for its activity and is present in a C. reinhardtii postribosomal supernatant as well as in a free polysome fraction. The latter could account for the observation that completion of nascent chains in free polysomes yielded S but not pS. We propose that pS is an extacloroplastic form of S and that the smallfpeptide portion plays a role in the transfer of S into the chloroplast.

Item Type: Article
Journal or Publication Title: Proc. Natl. Acad. Sci. USA
Volume: 74
Number: 3
Date Deposited: 10. Apr 2008 12:44
Date: 1977
Page Range: pp. 1082-1085
Faculties / Institutes: Service facilities > Center for Molecular Biology Heidelberg
Subjects: 570 Life sciences
Schriftenreihe ID: Works by Bernhard Dobberstein
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