The EMBO Journal , 4 (9). pp. 2153-2158.
The secondary structure of zein mRNA affects its translational potential. Here we show that in a cell-free system the translation efficiency of zein mRNA containing inverted repeats in the 5'- and 3'-untranslated regions is reduced. This translational block is released after deletion of the 3'-inverted repeat. We conclude that the translational block is caused by hybrid formation between the two inverted repeats. The translational efficiency of zein mRNAs, is also affected by varying the length or the primary structure of the 5'-untranslated region.
|Journal or Publication Title:||The EMBO Journal|
|Page Range:||pp. 2153-2158|
|Faculties / Institutes:||Service facilities > Center for Molecular Biology Heidelberg|
|Subjects:||570 Life sciences|
|Uncontrolled Keywords:||in vitro transcription-translation , zein mRNA , inverted repeats , 5'- and 3'-untranslated regions , translational potential|
|Schriftenreihe ID:||Works by Bernhard Dobberstein|