Directly to content
  1. Publishing |
  2. Search |
  3. Browse |
  4. Recent items rss |
  5. Open Access |
  6. Jur. Issues |
  7. DeutschClear Cookie - decide language by browser settings

Requirements for the Membrane Insertion of Signal-anchor Type Proteins

High, Stephen ; Flint, Nicholas ; Dobberstein, Bernhard

In: The Journal of Cell Biology, 113 (1991), Nr. 1. pp. 25-34

[thumbnail of High_1991a_JCB.pdf]
Preview
PDF, English
Download (2MB) | Terms of use

Citation of documents: Please do not cite the URL that is displayed in your browser location input, instead use the DOI, URN or the persistent URL below, as we can guarantee their long-time accessibility.

Abstract

Proteins which are inserted and anchored in the membrane of the ER by an uncleaved signalanchor sequence can assume two final orientations. Type I signal-anchor proteins translocate the NH2 terminus across the membrane while type 1I signalanchor proteins translocate the COOH terminus. We investigated the requirements for cytosolic protein components and nucleotides for the membrane targeting and insertion of single-spanning type I signalanchor proteins. Besides the ribosome, signal recognition particle (SRP), GTE and rough microsomes(RMs) no other components were found to be required. The GTP analogue GMPPNP could substitute for GTP in supporting the membrane insertion of IMC-CAT. By using a photocrosslinking assay we show that for secreted, type I and type II signalanchor proteins the presence of both GTP and RMs is required for the release of the nascent chain from the 54-kD subunit of SRP. For two of the proteins studied the release of the nascent chain from SRP54 was accompanied by a new interaction with components of the ER. We conclude that the GTP-dependent release of the nascent chain from SRP54 occurs in an identical manner for each of the proteins studied.

Document type: Article
Journal or Publication Title: The Journal of Cell Biology
Volume: 113
Number: 1
Date Deposited: 15 Jul 2008 17:30
Date: 1991
Page Range: pp. 25-34
Faculties / Institutes: Service facilities > Center for Molecular Biology Heidelberg
DDC-classification: 570 Life sciences
Series: Works by Bernhard Dobberstein
About | FAQ | Contact | Imprint |
OA-LogoDINI certificate 2013Logo der Open-Archives-Initiative