Directly to content
  1. Publishing |
  2. Search |
  3. Browse |
  4. Recent items rss |
  5. Open Access |
  6. Jur. Issues |
  7. DeutschClear Cookie - decide language by browser settings

The methionine-rich domain of the 54 kDa subunit of signal recognition particle is sufficient for the interaction with signal sequences

Lütcke, Henrich and High, Stephen and Römisch, Karin and Ashford, Anthony J. and Dobberstein, Bernhard

In: The EMBO Journal, 11 (1992), Nr. 4. pp. 1543-1551

[img]
Preview
PDF, English
Download (2352Kb) | Terms of use

Citation of documents: Please do not cite the URL that is displayed in your browser location input, instead use the persistent URL or the URN below, as we can guarantee their long-time accessibility.

Abstract

The signal recognition particle (SRP) binds to signal sequences when they emerge from a translating ribosome and targets the complex of ribosome, nascent chain and SRP to the membrane of the rough endoplasmic reticulum (rER) allowing the co-translational translocation of the nascent chain. By photo-crosslinking it has been shown that the signal sequence of preprolactin(PPL) only interacts with the methionine-rich (NI) domain of the 54 kDa protein subunit (SRP54) of SRP. Here we show that (i) a signal-anchor sequence is likewise crosslinked only to the methionine-rich domain of SRP54,(ii) free SRP54 can interact with signal sequences independently of the other components of SRP, (iii) its M domain suffices to perform this function, and (iv) an essentially intact M domain is required for signal sequence recognition. Alkylation of the N+G domain in intact SRP54 with N-ethyl maleimide (NE), but not after cleavage with V8 protease, prevents the binding of a signal sequence to the M domain. This suggests a proximity between the N+G and M domains of SRP54 and raises the possibility that the role of the N+G domain may be to regulate the binding and/or the release of signal sequences.

Item Type: Article
Journal or Publication Title: The EMBO Journal
Volume: 11
Number: 4
Date Deposited: 15. Jul 2008 17:26
Date: 1992
Page Range: pp. 1543-1551
Faculties / Institutes: Service facilities > Center for Molecular Biology Heidelberg
Subjects: 570 Life sciences
Uncontrolled Keywords: GTP binding protein , methionine-rich domain of SRP54 , photo-crosslinking , signal sequence binding , signal recognition particle
Schriftenreihe ID: Works by Bernhard Dobberstein
About | FAQ | Contact | Imprint |
OA-LogoLogo der Open-Archives-Initiative