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Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence

High, Stephen and Martoglio, Bruno and Görlich, Dirk and Andersen, Soren S. L. and Ashford, Anthony J. and Giner, Angelika and Hartmann, Enno and Prehn, Siegfried and Rapoport, Tom A. and Dobberstein, Bernhard and Brunner, Josef

In: The Journal of Biological Chemistry, 268 (1993), Nr. 35. pp. 26746-2671.

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Abstract

A chemically charged amber suppressor tRNA was used to introduce the photoactivatable amino acid (Tmd)Phe at a selected position within the signal sequence of the secretory protein preprolactin. This allowed the interactions of the NH -terminal, the central, and the COOH-terminal regions of the signal sequence to be investigated during insertion into the membrane of the endoplasmic reticulum (ER). We found that different regions of the nascent chains were photocross-linked to different ER proteins. The TRAM protein (translocating chain-associating membrane protein) contacts the NHz-terminal region of the signal sequence while the mammalian Sec6lp contacts the hydrophobic core of the signal sequence and regions COOH-terminal of this. These results suggest that the ER translocation complex is composed of heterologous protein subunits which contadcti stinct regions of nascent polypeptides during their membrane insertion.

Item Type: Article
Journal or Publication Title: The Journal of Biological Chemistry
Volume: 268
Number: 35
Date Deposited: 17. Jul 2008 17:10
Date: 1993
Page Range: pp. 26746-2671
Faculties / Institutes: Service facilities > Center for Molecular Biology Heidelberg
Subjects: 570 Life sciences
Schriftenreihe ID: Works by Bernhard Dobberstein
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