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Site-specific Photocross-linking Reveals That SecGlp and TRAM Contact Different Regions of a Membrane-inserted Signal Sequence

High, Stephen ; Martoglio, Bruno ; Görlich, Dirk ; Andersen, Soren S. L. ; Ashford, Anthony J. ; Giner, Angelika ; Hartmann, Enno ; Prehn, Siegfried ; Rapoport, Tom A. ; Dobberstein, Bernhard ; Brunner, Josef

In: The Journal of Biological Chemistry, 268 (1993), Nr. 35. pp. 26746-2671

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A chemically charged amber suppressor tRNA was used to introduce the photoactivatable amino acid (Tmd)Phe at a selected position within the signal sequence of the secretory protein preprolactin. This allowed the interactions of the NH -terminal, the central, and the COOH-terminal regions of the signal sequence to be investigated during insertion into the membrane of the endoplasmic reticulum (ER). We found that different regions of the nascent chains were photocross-linked to different ER proteins. The TRAM protein (translocating chain-associating membrane protein) contacts the NHz-terminal region of the signal sequence while the mammalian Sec6lp contacts the hydrophobic core of the signal sequence and regions COOH-terminal of this. These results suggest that the ER translocation complex is composed of heterologous protein subunits which contadcti stinct regions of nascent polypeptides during their membrane insertion.

Item Type: Article
Journal or Publication Title: The Journal of Biological Chemistry
Volume: 268
Number: 35
Date Deposited: 17 Jul 2008 17:10
Date: 1993
Page Range: pp. 26746-2671
Faculties / Institutes: Service facilities > Center for Molecular Biology Heidelberg
Subjects: 570 Life sciences
Schriftenreihe ID: Works by Bernhard Dobberstein
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