In: The Journal of Biological Chemistry, 268 (1993), Nr. 35. pp. 26746-2671
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Abstract
A chemically charged amber suppressor tRNA was used to introduce the photoactivatable amino acid (Tmd)Phe at a selected position within the signal sequence of the secretory protein preprolactin. This allowed the interactions of the NH -terminal, the central, and the COOH-terminal regions of the signal sequence to be investigated during insertion into the membrane of the endoplasmic reticulum (ER). We found that different regions of the nascent chains were photocross-linked to different ER proteins. The TRAM protein (translocating chain-associating membrane protein) contacts the NHz-terminal region of the signal sequence while the mammalian Sec6lp contacts the hydrophobic core of the signal sequence and regions COOH-terminal of this. These results suggest that the ER translocation complex is composed of heterologous protein subunits which contadcti stinct regions of nascent polypeptides during their membrane insertion.
Document type: | Article |
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Journal or Publication Title: | The Journal of Biological Chemistry |
Volume: | 268 |
Number: | 35 |
Date Deposited: | 17 Jul 2008 17:10 |
Date: | 1993 |
Page Range: | pp. 26746-2671 |
Faculties / Institutes: | Service facilities > Center for Molecular Biology Heidelberg |
DDC-classification: | 570 Life sciences |
Series: | Works by Bernhard Dobberstein |