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Structure and biosynthesis of the signal-sequence receptor

Prehn, Siegfried ; Herz, Joachim ; Hartmann, Enno ; Kurzchalia, Teymuras V. ; Frank, Rainer ; Römisch, Karin ; Dobberstein, Bernhard ; Rapoport, Tom A.

In: European Journal of Biochemistry, 188 (1990), pp. 439-445

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The signal-sequence receptor (SSR) has previously been shown to be a component of the environment which nascent polypeptides meet on passage through the endoplasmic reticulum (ER) membrane. We report here on the primary structure of the SSR as deduced from cDNA clones and from direct protein sequencing. The glycoprotein is synthesized with a cleavable amino-terminal signal sequence and contains only one classical membrane-spanning segment. Its insertion into the ER membrane during biosynthesis depends on the function of the signal-recognition particle. SSR shows a remarkable charge distribution with the amino terminus being highly negatively charged, and the cytoplasmic carboxyl terminus positively charged. The SSR can be phosphorylated in its cytoplasmic tail both in intact cells and in a cell-free system, suggesting a regulation of its function. The localization of the protein in the ER membrane was confirmed by immunofluorescence microscopy.

Item Type: Article
Journal or Publication Title: European Journal of Biochemistry
Volume: 188
Date Deposited: 02 Mar 2009 10:59
Date: 1990
Page Range: pp. 439-445
Faculties / Institutes: Service facilities > Center for Molecular Biology Heidelberg
Subjects: 570 Life sciences
Schriftenreihe ID: Works by Bernhard Dobberstein
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