Identification of an H-2Kd gene using a specific cDNA probe

Xin, J.-H. and Kvist, S. and Dobberstein, B.

The EMBO Joumal, 1 (4). pp. 467-471.

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Abstract

A cDNA clone known to code for a mouse histocompatibility (class I) antigen was found to contain a sequence specific for a subpopulation of H-2 genes. This unique sequence is located in the 3' non-coding region close to the stretch of poly(A) nucleotides. A subclone containing this fragment(pH-2d-5) has been used to select hybridizing mRNA. Translation of the mRNA in vitro shows that H-2Kd mRNA is selected. Southern blot analysis of DNA from congenic recombinant mice show that at least one gene containing this sequence is located at the K locus (region) of the major histocompatibility complex. This gene contains a 3.7-kb Bgml and a 13-kb EcoRI restriction endonuclease fragment. This gene has been isolated from a genomic DNA library.

Item Type:	Article
Journal or Publication Title:	The EMBO Joumal
Volume:	1
Number:	4
Date:	1982
Page Range:	pp. 467-471
Faculties / Institutes:	Service facilities > Center for Molecular Biology Heidelberg
Subjects:	570 Life sciences
Uncontrolled Keywords:	histocompatibility genes , hybrid-selected translation , gene mapping , 3' non-coding region, Southern blot analysis
Schriftenreihe ID:	Works by Bernhard Dobberstein