In: The EMBO Joumal, 1 (1982), Nr. 4. pp. 467-471.
A cDNA clone known to code for a mouse histocompatibility (class I) antigen was found to contain a sequence specific for a subpopulation of H-2 genes. This unique sequence is located in the 3' non-coding region close to the stretch of poly(A) nucleotides. A subclone containing this fragment(pH-2d-5) has been used to select hybridizing mRNA. Translation of the mRNA in vitro shows that H-2Kd mRNA is selected. Southern blot analysis of DNA from congenic recombinant mice show that at least one gene containing this sequence is located at the K locus (region) of the major histocompatibility complex. This gene contains a 3.7-kb Bgml and a 13-kb EcoRI restriction endonuclease fragment. This gene has been isolated from a genomic DNA library.
|Journal or Publication Title:||The EMBO Joumal|
|Date Deposited:||02 Mar 2009 11:01|
|Page Range:||pp. 467-471|
|Faculties / Institutes:||Service facilities > Center for Molecular Biology Heidelberg|
|Subjects:||570 Life sciences|
|Uncontrolled Keywords:||histocompatibility genes , hybrid-selected translation , gene mapping , 3' non-coding region, Southern blot analysis|
|Schriftenreihe ID:||Works by Bernhard Dobberstein|