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Identification of an H-2Kd gene using a specific cDNA probe

Xin, J.-H. and Kvist, S. and Dobberstein, B.

In: The EMBO Joumal, 1 (1982), Nr. 4. pp. 467-471.

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Abstract

A cDNA clone known to code for a mouse histocompatibility (class I) antigen was found to contain a sequence specific for a subpopulation of H-2 genes. This unique sequence is located in the 3' non-coding region close to the stretch of poly(A) nucleotides. A subclone containing this fragment(pH-2d-5) has been used to select hybridizing mRNA. Translation of the mRNA in vitro shows that H-2Kd mRNA is selected. Southern blot analysis of DNA from congenic recombinant mice show that at least one gene containing this sequence is located at the K locus (region) of the major histocompatibility complex. This gene contains a 3.7-kb Bgml and a 13-kb EcoRI restriction endonuclease fragment. This gene has been isolated from a genomic DNA library.

Item Type: Article
Journal or Publication Title: The EMBO Joumal
Volume: 1
Number: 4
Date Deposited: 02 Mar 2009 11:01
Date: 1982
Page Range: pp. 467-471
Faculties / Institutes: Service facilities > Center for Molecular Biology Heidelberg
Subjects: 570 Life sciences
Uncontrolled Keywords: histocompatibility genes , hybrid-selected translation , gene mapping , 3' non-coding region, Southern blot analysis
Schriftenreihe ID: Works by Bernhard Dobberstein
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