In: The EMBO Joumal, 1 (1982), Nr. 4. pp. 467-471
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Abstract
A cDNA clone known to code for a mouse histocompatibility (class I) antigen was found to contain a sequence specific for a subpopulation of H-2 genes. This unique sequence is located in the 3' non-coding region close to the stretch of poly(A) nucleotides. A subclone containing this fragment(pH-2d-5) has been used to select hybridizing mRNA. Translation of the mRNA in vitro shows that H-2Kd mRNA is selected. Southern blot analysis of DNA from congenic recombinant mice show that at least one gene containing this sequence is located at the K locus (region) of the major histocompatibility complex. This gene contains a 3.7-kb Bgml and a 13-kb EcoRI restriction endonuclease fragment. This gene has been isolated from a genomic DNA library.
Document type: | Article |
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Journal or Publication Title: | The EMBO Joumal |
Volume: | 1 |
Number: | 4 |
Date Deposited: | 02 Mar 2009 11:01 |
Date: | 1982 |
Page Range: | pp. 467-471 |
Faculties / Institutes: | Service facilities > Center for Molecular Biology Heidelberg |
DDC-classification: | 570 Life sciences |
Uncontrolled Keywords: | histocompatibility genes , hybrid-selected translation , gene mapping , 3' non-coding region, Southern blot analysis |
Series: | Works by Bernhard Dobberstein |