In: Journal of Translational Medicine, 16 (2018), Nr. 50. S. 1-7. ISSN 1479-5876
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Abstract
Background; CD133 is a marker of stem cells as well cancer stem cells. This study investigated the association between CD133 expression in cancer cells and the clinical outcome of non-mucin producing intrahepatic cholangiocarcinoma (ICC).
Methods: Fifty-seven non-mucin producing ICC patients were enrolled in this study. Immunohistochemistry (IHC) and immunofluorescence staining for CD133 as well as other cancer-associated proteins, including cytokeratin 19, TGF-β1, p-Smad2 and epithelial–mesenchymal transition (EMT) markers S100A4, E-Cadherin and Vimentin were analyzed.
Results: IHC staining showed that tumor cells in 52.6% of patients expressed CD133. The CD133+ patients had significantly higher metastasis rate than those without CD133+ tumor cells (36.7% vs. 10.1%, p = 0.03). The CD133+ patients had shorter overall and disease-free survival time as compared to the CD133− patients. Furthermore, 90.9% of CD133+ patients developed cancer recurrence, as compared to 64.3% of CD133− patients (p = 0.02). As compared to CD133− patients, tumor cells in CD133+ patients demonstrated high levels of TGF-β/p-Smad2 as well as EMT-like alteration, characterized by loss of E-Cadherin and expression of Vimentin and S100A4.
Conclusions: CD133 expression in ICC tumor cells indicates poor prognosis of the disease and might be associated with TGF-β related EMT alterations.
Dokumententyp: | Artikel |
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Titel der Zeitschrift: | Journal of Translational Medicine |
Band: | 16 |
Nummer: | 50 |
Verlag: | BioMed Central |
Ort der Veröffentlichung: | London |
Erstellungsdatum: | 27 Apr. 2018 09:54 |
Erscheinungsjahr: | 2018 |
ISSN: | 1479-5876 |
Seitenbereich: | S. 1-7 |
Institute/Einrichtungen: | Medizinische Fakultät Mannheim > Medizinische Klinik - Lehrstuhl für Innere Medizin II |
DDC-Sachgruppe: | 610 Medizin |