In: Proc. Natl. Acad. Sci. USA, 74 (1977), Nr. 3. S. 1082-1085
Vorschau |
PDF, Deutsch
Download (1MB) | Nutzungsbedingungen |
Abstract
Translation of polyadenylated mRNA of Chlamydomonas reinhardtii in a cell-free wheat germ system resulted in the synthesis of numerous discrete polypeptides. Among them was a species with molecular weight 20,000 that was immunoprecipitated specifically by antibodies raised against the authentic small subunit (16,500 daltons) of ribulose-1,5-bisphosphate carboxylase [3-phospho-D-glycerate carboxy-lyase(dimerizing), EC 4.1.1.39]. Since the immunoprecipitated polypeptide has a larger molecular weight by approximately 3500 than the small subunit (S) it was identified as a putative biosynthetic precursor (pS). Post-translational conversion of pS by a specific endoprotease yielded two detectable products: one apparently identical in size to S and the other, a small peptide, presumably representing the remainder of pS. The endoprotease requires sulfhydryl groups for its activity and is present in a C. reinhardtii postribosomal supernatant as well as in a free polysome fraction. The latter could account for the observation that completion of nascent chains in free polysomes yielded S but not pS. We propose that pS is an extacloroplastic form of S and that the smallfpeptide portion plays a role in the transfer of S into the chloroplast.
Dokumententyp: | Artikel |
---|---|
Titel der Zeitschrift: | Proc. Natl. Acad. Sci. USA |
Band: | 74 |
Nummer: | 3 |
Erstellungsdatum: | 10 Apr. 2008 12:44 |
Erscheinungsjahr: | 1977 |
Seitenbereich: | S. 1082-1085 |
Institute/Einrichtungen: | Zentrale und Sonstige Einrichtungen > Zentrum für Molekulare Biologie (ZMBH) |
DDC-Sachgruppe: | 570 Biowissenschaften, Biologie |
Schriftenreihe: | Schriften von Bernhard Dobberstein |